I have query regarding tool for de novo assembly of PacBio data. I have plant genome data at 52X coverage. The genome size is around 500 mb
I used HGAP (RS_HGAP_assembly2, RS_HGAP_assembly3 , RS_preassembly 2) tool through SMRT portal it giving me error of "ERROR! Reading fasta files greater than 4Gbytes is not supported" . It is not supporting large gnome size
Then I used falcon it run successfully but in assembly folder the all files are empty except preads.ovl, 2-asm-falcon/run_falcon_asm.sh.log
Can you please suggest me tool for assembly for data having 52x coverage and predicted genome size is 500mb .