Forum: Gallery: Computational tools for DNA methylation
8
gravatar for Shicheng Guo
21 months ago by
Shicheng Guo4.4k
Shicheng Guo4.4k wrote:

More suggestions? 

BeadScan: Analysing data from Illumina BeadArrays
BisReadMapper: Library-free methylation sequencing with bisulfite padlock 
BRAT: Highly configurable and well-documented three‑letter bisulphite aligner
BSMAP: Probably the most widely used wild-card bisulphite aligner
BSmooth is a pipeline for analyzing WGBS data: aligning, quality control, and identifying DMRs.
ComBat: R script for correcting known or suspected batch effects 
DMAP: differential methylation analysis package for RRBS and WGBS data.
GenomeStudio: GenomeStudio Methylation Module v1.8
GSNAP: Wild-card bisulphite aligner included in a widely used general-purpose
isva: R package for batch effect correction using an algorithm
MethylCoder: Three‑letter bisulphite aligner that can be used with either Bowtie and Bowtie2 
methylumi: R/Bioconductor package for Infinium data normalization
minfi: R/Bioconductor package for Infinium data normalization, analysis
Pash: Wild-card bisulphite aligner included in a general-purpose alignment tool 
RMAP: Wild-card bisulphite aligner included in a general-purpose alignment tool
RnBeads: R package providing a software pipeline for Infinium data normalization,
RRBSMAP: Variant of BSMAP that is specialized on reduced-representation
segemehl: Wild-card bisulphite aligner included in a general-purpose alignment tool
SVA: R/Bioconductor package for correcting batch effects
methyLiftover: cross-platform DNA methylation data integration
dariober's methylation-cafe: https://github.com/dariober/bioinformatics-cafe
metilene for DMR (Fast and Sensitive: better than MOABS and Bsmooth)http://www.bioinf.uni-leipzig.de/Software/metilene/Downloads/
scLVM: single-cell RNA-seq heterogeneity source deconvolution
methpipe: http://smithlabresearch.org/software/methpipe/
Bis-SNP: http://people.csail.mit.edu/dnaase/bissnp2011/
COMETs: Information recovery from low coverage whole-genome bisulfite sequencing
HMMcopy: copy number estimations for whole genome data with GC and mappability correction
BS-SNPer: SNP calling in bisulfite-seq data

ADD COMMENTlink modified 9 days ago by william.bill.langdon30 • written 21 months ago by Shicheng Guo4.4k
6
gravatar for David Langenberger
13 months ago by
Deutschland
David Langenberger7.9k wrote:

metilene (Link)

References:

  • Jühling F, Kretzmer H, Bernhart SH, Otto C, Stadler PF, Hoffmann S., "metilene: fast and sensitive calling of differentially methylated regions from bisulfite sequencing data.", Genome Res. 2016 Feb;26(2):256-62.
  • Kretzmer et al., "DNA methylome analysis in Burkitt and follicular lymphomas identifies differentially methylated regions linked to somatic mutation and transcriptional control.", Nat Genet. 2015 Nov;47(11):1316-25.
ADD COMMENTlink written 13 months ago by David Langenberger7.9k

DNA methylation needs 5 stages: Mapping and Normalization Preprocessing Analysing Visualisation Validation and interpretation Partek company has a software for all these stages but it is not free. My question is: 1- Which of the current packages have the five stages with different options? 2- I need a tutorial to follow to reproduce some figures like? Thank you

ADD REPLYlink written 8 months ago by forever50
4
gravatar for Antonio R. Franco
21 months ago by
Spain. Universidad de Córdoba
Antonio R. Franco3.4k wrote:

Bismark?

BiSeq (under R)

 

 

ADD COMMENTlink modified 21 months ago • written 21 months ago by Antonio R. Franco3.4k
3
gravatar for Sandeep
21 months ago by
Sandeep230
Manipal, India
Sandeep230 wrote:

dna-bison - Bisulfite alignment On Nodes of a cluster

NextGenMap - Has a methylation specific mapper

bwa-meth - Align BS-Seq reads and extract methylation without intermediate temp files

BiQ analyser Easy visualization and quality control of DNA methylation

 

 

 

ADD COMMENTlink written 21 months ago by Sandeep230
3
gravatar for dariober
16 months ago by
dariober8.0k
Glasgow - UK
dariober8.0k wrote:

...Since this thread as returned to the top, here's my 2p.

I wrote a simple parser to extract methylation from bam files, bam2methylation.py

ADD COMMENTlink written 16 months ago by dariober8.0k
2
gravatar for william.bill.langdon
8 months ago by
william.bill.langdon30 wrote:

The latest version of BarraCUDA (ie 0.7.107f http://sourceforge.net/projects/seqbarracuda/, effectively BWA on CUDA hardware) can be used with epigenetics NGS data on graphics cards. However note that currently epigenetics data effectively doubles the size of the memory needed for the reference genome, so for human data you may need a GPU with more than 6GB. For noisy data, you might want to increase the allowed number of mismatches (option -n). Bill

ADD COMMENTlink written 8 months ago by william.bill.langdon30
1
0
gravatar for william.bill.langdon
8 months ago by
william.bill.langdon30 wrote:

New technical report RN/16/10 https://doi.org/10.1101/095075 gives BarraCUDA performance on nvidia Titan X (pascal), GTX 1080 and Titan X (compute level 5.2) graphics cards on paired end Cambridge Epigenetix data. Bill

ADD COMMENTlink written 8 months ago by william.bill.langdon30
0
gravatar for william.bill.langdon
9 days ago by
william.bill.langdon30 wrote:

BioData Mining has just published our "Short Report" on Genetically improved BarraCUDA. Bill

ADD COMMENTlink written 9 days ago by william.bill.langdon30
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