As title suggests, I have designed primers for a gene (BRCA1) with help of ExonPrimer using hg19 as template. Problem is that in order to make manifest file in Illumina Experiment Manager (IEM) for PCR Amplicon Workflow, I need to have hg19 start and end co-ordinates of my amplicons but I have not been suplied this bit of information. So, is there any way of getting co-ordinates with help of forward and reverse primer sequences?
For Example, I have been supplied this bit of information for exon1 of BRCA1 gene,
Feb. 2009 (GRCh.37/hg19)
Homo sapiens breast cancer 1, early onset (BRCA1), transcript variant 1, mRNA