Hi all, Im not sure if my previous post on the same topic was posted right. so here I go again.
I have tried re-ordering 454 derived contigs from the bacterial genome Im working with, using mauve (with one reference genome). however, I would now like to use multiple reference genomes to reduce gaps and ultimately the number of PCRs that may need to be done to stitch things together.
is anyone aware of a program that would let us do this? I have tried PGA4genomics web server with little success.
any input greatly appreciated. thanks guys.