I did an sequencing run where I enriched specific DNA regions. Thus I expect to have a lot of PCR duplicates. In the figure below you can see a IGV print screen of a specific region. You can see that pretty all reads are the same. But several of them has some mismatches (see arrows) (less than 1% of the reads have some mismatches). Can I consider that they are PCR duplicates ? or are they real different DNA fragments ?