Question: Compare 450k with EPIC
gravatar for Tanvir Ahamed
2.8 years ago by
Tanvir Ahamed 270 wrote:

I have the idat file form a same sample in 450k and EPIC.

What is the best way to compare those results?

Can i compare raw data or should I apply any specific normalization before comparison?

epic 850k methylation 450k • 3.4k views
ADD COMMENTlink modified 23 months ago by joshmoss110 • written 2.8 years ago by Tanvir Ahamed 270
gravatar for fortin946
2.8 years ago by
United States/Baltimore/Johns Hopkins University
fortin946180 wrote:

We have now tested multiple functionalities of the minfi package for the analysis of EPIC arrays. We have performed a comparison of the different normalization methods in minfi, and the results are presented in our recent preprint:

Hope this helps,


ADD COMMENTlink written 2.8 years ago by fortin946180
gravatar for Charles Warden
2.8 years ago by
Charles Warden6.6k
Duarte, CA
Charles Warden6.6k wrote:

You would apply some sort of normalization.

Some some normalization strategies may work better for 450k vs EPIC arrays (for example, the proportion of Type I vs Type II probes is different), but I've found that the Illumina normalization seems to work pretty well in both cases.

You can apply Illumina normalization within Genome Studio, or the minfi Bioconductor package using preprocessIllumina(). This applies a background adjustment and normalization to controls.

It would also be best to have multiple samples to compare differential methylation (at the site or region level). For example, if you apply a delta beta threshold of 0.2, that might be greater than the difference in beta values obtained from different normalization methods (although it could affect marginally significant candidates).

ADD COMMENTlink modified 2.8 years ago • written 2.8 years ago by Charles Warden6.6k

Thanks for your suggestion .

Can you please tell me, it it possible to get the same result (equivalent value) for intensity on common probe for 450k and EPIC. So for that, can i compare raw data or any further normalization or other procedure if required ?

ADD REPLYlink written 2.8 years ago by Tanvir Ahamed 270
gravatar for maureenash262
2.8 years ago by
maureenash2620 wrote:

The minfi package provides tools for analyzing Illumina's Methylation arrays, specifically the 450k and EPIC (also known as the 850k) arrays.Arrays offer a reliable cost effective way of analysing multiple targets on a large scale. The Genome Centre has offered Illumina methylation arrays since their original golden gate assay. Every student will get any type of academic paper and writing tips from the different resources and make his/her educational life run more smoothly and effectively.

ADD COMMENTlink written 2.8 years ago by maureenash2620

Thank you very much:) It became more clearly. I think that appropriate tools can really change the situation in a good way. I'm glad that you provided that information:)

ADD REPLYlink written 2.1 years ago by svqw0
gravatar for joshmoss11
23 months ago by
joshmoss110 wrote:

I have found many differences between results from EPIC and 450k even after performing illumina normalization. Has anyone else come across this/have any ideas how to deal with this?

ADD COMMENTlink written 23 months ago by joshmoss110

Check this paper from minfi developers:

We discuss methods for the joint analysis and normalization of data from the HumanMethylation450 (‘450k’) and EPIC platforms.

ADD REPLYlink written 23 months ago by igor7.6k
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