Question: How to get multiple alignments from different species using lastz?
0
gravatar for Cacau
3.0 years ago by
Cacau450
Canada
Cacau450 wrote:

For example, I want to perform multiple genome alignment for cat, dog and cow using lastz. How should I specify the argument to run alignment for all of them?

Or should I run pairwise lastz and then combine the results? If so, how should I do?

alignment • 1.1k views
ADD COMMENTlink modified 13 months ago by Biostar ♦♦ 20 • written 3.0 years ago by Cacau450

Lastz is a pair-wise aligner.

Have you looked at the pre-computed alignments from UCSC (scroll down the page and find multiple-alignments) or Ensembl (these are pairwise alignments with synteny analysis for some) for vertebrate genomes?

ADD REPLYlink modified 3.0 years ago • written 3.0 years ago by genomax71k

Pre-computed alignments are not available for domestic water buffalo (Bubalus bubalis) in UCSC or ensemble. I want to create a maf file for buffalo with human, cow and dog. Is there any tutorial links on how to perform multiple alignment on genomes? What may be memory requirement to do the same?

ADD REPLYlink written 4 weeks ago by kousi310
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