I generated an annotated transcriptome of a non model organism using Trinity and Trinotate. I would like to perform some GO terms enrichment analysis. I did this a month ago on the website of wego (http://wego.genomics.org.cn/) but it's not working anymore. All the other alternatives I checked (AMIGO, goProfiles ...) use proper gene annotation as starting point but I only have the GO terms associated with my transcript. Is there a way to do the analysis starting with the file I have.
Here is an examples of my files:
transcript GO term
TR35016|c140_g012080 GO:0005576; GO:0005319
TR8218|c4_g015662 GO:0005576; GO:0005319
TR9751|c8_g015821 GO:0005576; GO:0005319
TR27091|c6_g010849 GO:0016021; GO:0005743; GO:0070469; GO:0004129; GO:0020037; GO:0005506; GO:0009060
TR24288|c6_g010207 GO:0005743; GO:0045275; GO:0009055; GO:0046872; GO:0008121
TR8216|c3_g015661 GO:0005576; GO:0005319
TR35068|c18_g012086 GO:0005576; GO:0005319
TR31387|c2_g011562 GO:0005576; GO:0005319
Thank you for your help.