My plan is to develop a tool that can predict gene mutation and gene's expression pattern in a user input sample (fastq). From literature survey, I have understood Exome seq is good for calling variants from sample as it call variants from all coding region and RNA-Seq is best for gene / transcript expression data analysis. But, people do go for variant analysis using RNA-seq also. In such case all genomic variants are not captured. Here I have three important question
- Can I use RNAseq for identifying the variants for clinical analysis?
- Is RNA-Seq clinically more important than exome seq as it is used for both variant calling and identifying transcript expression?
- Are Mutations in expressed regions more important than mutations in non-expressed region? Please give your valuable suggestions.
If you need any clarification in the question please reply me here.
Sorry for my language.