Question: Tophat software error
2.1 years ago by
saj98 • 100
saj98 • 100 wrote:
Hello every one I used the following tophat command to get thout file.
tophat -p 5 -o WS001_R1_thout --library-type=fr-firststrand -G Documents/Shaima/Seq/Pros/S/Rn6/Rn6.gtf Documents/Shaima/Seq/Pros/S/Rn6/Rn6_index Documents/Shaima/Seq/Pros/S/WS020_S6_L002_R1_001.fastq Documents/Shaima/Seq/Pros/S/WS020_S6_L002_R2_001.fastq
The program run successfully, but then gave me the following error:
Error: failed to retrieve right read for pair # 8885216 !
Appreciate your help
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