I have some RNAseq data and trying to align them to the genome (hg19) using both STAR and BWA separately. but the problem is that for each file it should take at least half an hour but the strange thing is that for these files it takes at most 6 minutes and at the end when I visualize the bam files on UCSC or IGV for many genes I do not have enough reads (in fact very few reads which is not really normal based on my experience). do you know what the possible problem is?
Question: problem with the alignment of RNAseq data
2.7 years ago by
Sara • 90
Sara • 90 wrote:
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