have RNAseq data from 9 different plant samples, different cultivars of one species. I have a table of miRNA read counts for each and would like to compare the counts(expression) of these miRNAs. Can I use DESeq2 for that?
I am essentially a non-programmer, got as far as installing the DESeq2 package in R, but the instructions on how to set up the DESeqDataSet from my excel-txt-exported table leave me completely confused, in part because I do not have timepoints or conditions. I am at a tiny university outpost, so there is nobody I can ask questions about this, any help that tells me specifically what steps are needed for my specific table would be very ,very greatly appreciated. nor AMP1 shv shc vvi-miR156 206 256 209 215 vvi-miR159 100 100 100 100 vvi-miR160 0 2 100 105