Hello, I have computed the coverage of my reads with bamQC and I got, for one of my reads:
>>>>>>> Coverage mean coverageData = 33.5984X std coverageData = 4,599.979X There is a 1,3% of reference with a coverageData >= 1X There is a 1,14% of reference with a coverageData >= 2X There is a 0,85% of reference with a coverageData >= 3X
This 33X is good for rnaseq analysis, but seeing the std so big and a very low percentage of reference above 3X, seems very bad. What should my conclusion be? Even if its a high mean coverage if it has a standard deviation so high, then this should be a bad coverage? Or is this mean coverage still good even if I have a huge std?
Please, help me interpret these results.