Question: Peptide Blast on MS/MS Spectra Databases
0
gravatar for b.verhagen
6 weeks ago by
b.verhagen0
b.verhagen0 wrote:

Hi,

I was wondering whether the peptide sequences that correspond to mass spectra, deposited in spectra databases, can be obtained somewhere?

Best regards, Bram Verhagen

ADD COMMENTlink written 6 weeks ago by b.verhagen0
1

I am not sure if Mascot is what you are looking for.

ADD REPLYlink written 6 weeks ago by genomax64k

Probably not I believe not (though I don't have much familiarity with MS/MS databases). If you can get the spectra and a protein database, you can estimate the protein abundances.

What "blast" has to do with your actual question?

ADD REPLYlink modified 6 weeks ago • written 6 weeks ago by h.mon24k

I don't think so.. as I would like to validate whether the spectrum, corresponding to the peptide sequence, is already present in the depository/database, prior to the real mass spec experiment.

ADD REPLYlink modified 6 weeks ago • written 6 weeks ago by b.verhagen0
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