Hello! I've de-novo assembled a transcriptome from Trinity, resulting into Trinity.fasta, whose headers look like this:
>TRINITY_DN29256_c0_g1_i1 len=323 path=[0:0-322]
Followed, in the next line, by the sequence.
To run an external downstream analysis with a R script, I'd need to have a .gff3 reference file (FeatureCounts function from RSubread). Of course, for now, annotation isn't needed, just names and coordinates.
I've already performed a classic edgeR analysis with Trinity, I'm just trying something different and need this very specific input file.
Can anyone help me here? Thanks in advance!