Tutorial:Getting Cegma/Genewise To Work On Centos, Rhel
2
5
Entering edit mode
7.5 years ago
Adrian Pelin ★ 2.4k
# This is based on a Ubuntu guide:

    http://korflab.ucdavis.edu/datasets/cegma/ubuntu_instructions_1.txt


# get these

    ftp://genome.crg.es/pub/software/geneid/geneid_v1.4.4.Jan_13_2011.tar.gz
    ftp://selab.janelia.org/pub/software/hmmer3/3.0/hmmer-3.0.tar.gz
    http://korflab.ucdavis.edu/Datasets/cegma/cegma_v2.4.010312.tar.gz
    ftp://ftp.ncbi.nlm.nih.gov/blast/executables/blast+/LATEST/ncbi-blast-2.2.25+-x64-linux.tar.gz
    http://ftp-stud.hs-esslingen.de/ubuntu/pool/universe/w/wise/wise_2.4.1.orig.tar.gz

# Genewise install

# need this to avoid glib-config error in genewise install

    sudo yum install glib
    sudo yum install glib-devel

    cd wise2.4.1/src

# modify makefile to use "CC = cc" into "CC = gcc"

    cd wise2.4.1/src/HMMer2/
    sed 's/getline/getline_new/' sqio.c  > a &&  mv a sqio.c

# that takes care of a get line error

    cd ../models/

# Change phasemodel.c
From:

    if( !isnumber(line[0]) ) {

To:

    if( !isdigit(line[0]) ) {

# That takes care of a isnumber error

# Genewise install is the most problematic one, the rest install flawlessly.

# I thought I would start from the Ubuntu guide and change what is needed for CentOS.Turns out the glib part is slightly different, the rest is the same.
tutorial Tutorial • 6.3k views
ADD COMMENT
1
Entering edit mode

This is a god-send :) Thank you!

ADD REPLY
2
Entering edit mode
6.6 years ago
Darked89 4.2k

re getting wise2.4.1:

  1. it exist as Debian package: https://packages.debian.org/unstable/science/wise
    • sudo apt-get install wise
  2. if you are not on Debian/Ubuntu, you will be better of getting wise_2.4.1-17.debian.tar.xz from the above Debian wise package page, unpacking it and applying to wise-2.4.1 from wise_2.4.1.orig.tar.gz by running:

    for file in debian/patches/[01]*.patch; do patch -p0 < $file; done
    

CAVEAT:

No idea why, but 10_fix_path_to_data_files.patch has different directory naming. Quick and dirty way of fixing it is to softlink wise-2.4.1 to b:

ln -s wise-2.4.1 b

Then everything compiled with gcc_4.8.1 without problems.

ADD COMMENT
0
Entering edit mode
7.3 years ago
Buttonwood ▴ 40

Does anyone know how to install genewise on a redhat OS server?

ADD COMMENT
0
Entering edit mode

above you is the exact tutorial on how to do it.

ADD REPLY
0
Entering edit mode

Thanks you very much

But I still need your help

I followed your steps

but get these:

make CC="gcc" CFLAGS="-c -O3 `glib-config --cflags`" libwisebase.a )
/bin/sh: glib-config: command not found

if test -x /bin/ranlib; then /bin/ranlib libdyna.a; else exit 0; fi
if test -x /usr/bin/ranlib; then /usr/bin/ranlib libdyna.a; else exit 0; fi 
make[1]: Leaving directory `/data/tanhao/dev/gene_prediction/GeneWise/wise-2.4.1/src/dynlibsrc'  
(cd dynlibsrc ; make CC="gcc" CFLAGS="-c -O3`glib-config --cflags`" libdyna_glib.a )  
/bin/sh: glib-config: command not found  
make[1]: Entering directory `/data/tanhao/dev/gene_prediction/GeneWise/wise-2.4.1/src/dynlibsrc'

I have done sudo yum install glib

Then I searched “/bin/sh: glib-config: command not found” and followed the seqanswer's tips: http://seqanswers.com/forums/showthread.php?t=24027

to edit the makefile and change

CFLAGS = -c -O3 `glib-config --cflags`

to

CFLAGS = -c -O3 `pkg-config --libs glib-2.0 --cflags`

But I still get errors like:

(cd network ; make  CC="gcc" CFLAGS="-c -O3 `pkg-config --libs glib-2.0 --cflags`" all ) 
make[1]: Entering directory `/data/tanhao/dev/gene_prediction/GeneWise/wise-2.4.1/src/network'
gcc -c -O3 -I/usr/include/glib-2.0 -I/usr/lib64/glib-2.0/include  -lglib-2.0   -I../base/  -I../socket -I../dynlibsrc -I../dnaindex   wise_proteinindex_server.c  
gcc -c -O3 -I/usr/include/glib-2.0 -I/usr/lib64/glib-2.0/include  -lglib-2.0   -I../base/  -I../socket -I../dynlibsrc -I../dnaindex net_hspscan.c  
gcc -g -o scanwise_server wise_proteinindex_server.o net_hspscan.o ../dnaindex/compressed_protein_index.o   ../dnaindex/kmer_index_interface.o ../dnaindex/singleseqspace.o ../dnaindex/kmer_direct.o  -ldyna_glib -ldyna -lwisesocket -lwisebase -g -L../base/ -L../socket -L../dynlibsrc -L../dnaindex -lm`glib-config --libs`-lpthread  
/bin/sh: glib-config: command not found  
../dynlibsrc/libdyna_glib.a(subseqhash.o): In function`Wise2_is_populated_subseqhash_ghash': 
subseqhash.c:(.text+0x18): undefined reference to `g_hash_table_lookup'  
../dynlibsrc/libdyna_glib.a(subseqhash.o): In function`Wise2_add_direct_number_subseqhash_ghash': 
subseqhash.c:(.text+0x7a): undefined reference to `g_hash_table_lookup'  
subseqhash.c:(.text+0xc2): undefined reference to `g_hash_table_insert'
../dynlibsrc/libdyna_glib.a(subseqhash.o): In function `Wise2_add_seq_subseqhash_ghash':  
subseqhash.c:(.text+0x138): undefined reference to `g_hash_table_lookup'
subseqhash.c:(.text+0x17a): undefined reference to `g_hash_table_insert'
../dynlibsrc/libdyna_glib.a(subseqhash.o): In function `Wise2_lookup_subseqhash_ghash':
subseqhash.c:(.text+0x1b8): undefined reference to `g_hash_table_lookup'

It seems the glib-config problem is still there!

Do you have any suggestion for me?And thank you again!

ADD REPLY
0
Entering edit mode

have you also done "sudo yum install glib-devel"?

ADD REPLY
0
Entering edit mode

Yes, I also done sudo yum install glib-devel but there is no such package;

so I did

find ./ -name make |xargs sed -i 's/glib-config/pkg-config --libs glib-2.0/'

and it seems work.

Thank you.

Darked89: ake changed to make

ADD REPLY

Login before adding your answer.

Traffic: 2239 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6