Question: Difference Between Gene Expression And Transcript Expression
5
gravatar for jack
5.2 years ago by
jack420
jack420 wrote:

What is the difference between gene expression and transcript expression ? for me both of them seems same.

expression transcript • 19k views
ADD COMMENTlink modified 5.2 years ago by Neilfws48k • written 5.2 years ago by jack420
12
gravatar for Floris Brenk
5.2 years ago by
Floris Brenk880
USA
Floris Brenk880 wrote:

Genes can have multiple transcripts. So gene expression could mean the overal expression of all transcripts of a gene. Transcript expression is the expression of a specific transcript. In the past microarray period gene expression was measured and the output was gene expression. Nowadays with next gen sequencing methods like RNA-seq, CAGE etc. specific transcript expression can be identified... Maybe read a bit about alternative splicing (http://en.wikipedia.org/wiki/Alternative_splicing)

ADD COMMENTlink modified 5.2 years ago • written 5.2 years ago by Floris Brenk880

to add: depending on how the gene is transcribed you get multiple alternative mRNA transcripts. Some transcripts are longer or shorter by one or more exons. Some mRNA transcripts further get spliced to be modified. Some transcripts could be expressed (translated) into mature proteins in specific tissue types and become dominant (http://www.ncbi.nlm.nih.gov/pubmed/8449945)

ADD REPLYlink modified 3.0 years ago • written 3.0 years ago by ammar.husami0
8
gravatar for Neilfws
5.2 years ago by
Neilfws48k
Sydney, Australia
Neilfws48k wrote:

In a sense, there is no such thing as a gene. A gene is a concept: a fuzzy bounded region of a chromosome within which transcription occurs. So really there is no gene expression, only transcript expression.

I'd even argue that "expression" itself is not a very useful term and we should simply use "transcription".

ADD COMMENTlink written 5.2 years ago by Neilfws48k
2
gravatar for Emily_Ensembl
5.2 years ago by
Emily_Ensembl17k
EMBL-EBI
Emily_Ensembl17k wrote:

It may also depend on the tools you're using. You may be able to determine that there is expression from a particular gene, but the tool you're using may not be able to differentiate between alternative splicing - in this case I would talk about gene expression. If I had more accurate tools, however, I might talk about transcript expression.

Eg, an RT-PCR using primers that both fall in the middle of a large constituent exon would give us info on gene expression. An RT-PCR using primers that spanned an intron junction that was specific one splice variant would give us info on transcript expression.

ADD COMMENTlink modified 5.2 years ago • written 5.2 years ago by Emily_Ensembl17k
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