Hi everyone, I am new in sequence assembly. I have stared a project of 50 ecoli whole genome sequencing illumina data set. I did all the assembly using Spades and quality checking by Quast. On an average i got around 100 contig of each genome. Can anyone suggest me how to improve the assembly quality, i mean how to reduce the contig number , increase the N50 value , reduce the gap?
Thank you advance for any suggestion.