Hello everyone, I am new here and new to BioInformatic analysis. I need some help, please. Currently, I have downloaded an RNAseq file from Cibioportal. The file was then pasted into Excel. So I have the patient IDs and different gene expression values. I want to pick a gene and group it into two groups (high and low). And based on these two groups, generate a VOLCANOPLOT and see which genes are up or down-regulated by following my gene expression. Thank you very much for your help.