Single Cell RNA Seq

Question

[scRNA2023]

Hi Members, I need to do an assignment in single cell RNA seq using the tools tagged above. I will appreciate if anyone can help me out. I have done basic scRNA analysis but need guidance on some of the tools.

Thank you in advance

[ATpoint]

Please understand that these open-ended Qs are difficult to answer. Read the manuals and help pages and then edit the Q with a specific problem.

[scRNA2023]

Hi ATpoint..

Thank you for the suggestion. I am familier with basic scRNAseq pipeline using Seurat. However, I am required to reanalyzed a published scRNA Seq data using the following tools: Doubletfinder, Harmony, Cell Typist, CellO, scGATE, Monocle, SoupX, Cellbender, scTransform

Hence, I wish to have a tutor who can help me out with this. I can be contacted at [e-mail] Thank you

[Ram]

Do not add answers unless you're answering the top level post. Use Add Comment or Add Reply instead as appropriate. I've moved your post to the right location this time.

[scRNA2023]

Thank you for this Ram. Highly appreciated.

[ATpoint]

Try something, then ask specific questions. It's not a code writing service here, nor do people actively contact you via email. That's just not how communities like this work.

[scRNA2023]

Hi ATpoint,

Yes I understand and I am trying to go through the documentations of respective packages also. However, I was just exploring if anyone might be interested to teach :-).

[Ram]

I was just exploring if anyone might be interested to teach :-).

Are you interesting in paying this someone that might be interested in teaching you?

[scRNA2023]

Yes of course..

[Ram]

There are free as well as paid courses online, why ask for this on a question you opened? Why not create a job posting with all relevant details? That will attract the users that freelance.

[scRNA2023]

yes.. you are right. I have seen similar posts here in Biostars also and though that i might find anyone willing to freelance..

[Kevin Blighe]

Those of us who reach the peak of our game invest much time in self-learning... driving ourselves each day to do better... to learn more.

There are many scRNA-seq tutorials available online for these tagged 'tools'. May you please conduct a search and then report back [here] with a bullet-point list of these tutorials - this would greatly assist the community.

[scRNA2023]

Hi, Yes sure, I can do a search and list down the tutorials for these tagged tools. But mostly, I am going through the documentations of these tools only.

[Kevin Blighe]

Thank you. May you have the report for me by Monday midday your local time? I would appreciate it. Thank you.

[scRNA2023]

What is the difference between the filtered and raw gene-barcode matrix?

[LChart]

This is well-earned: https://letmegooglethat.com/?q=10x+cellranger+filtered+vs+raw