Help with mauve

Question

[reachedahead225]

Hi I have downloaded my genomes and they wont work it comes as error and there is a gap in the alignment I will put it below

Running alignment.
Executing 
  mauveAligner
    --output=C:\Users\K1807836\Downloads\k-12
    --island-size=50
    --island-output=C:\Users\K1807836\Downloads\k-12.islands
    --backbone-size=50
    --max-backbone-gap=50
    --backbone-output=C:\Users\K1807836\Downloads\k-12.backbone
    --id-matrix=C:\Users\K1807836\Downloads\k-12.id_matrix
    --output-alignment=C:\Users\K1807836\Downloads\k-12.alignment
    --output-guide-tree=C:\Users\K1807836\Downloads\k-12.guide_tree
    C:\Users\K1807836\Downloads\Ecoli k-12 mg1655
    C:\Users\K1807836\Downloads\Ecoli k-12 mg1655.sslist
    C:\Users\K1807836\Downloads\Ecoli k-12 mg1655 Boston.gb
    C:\Users\K1807836\Downloads\Ecoli k-12 mg1655 Boston.gb.sslist
ERROR! gap character encountered at genome sequence position 21Sequence file appears corrupt, proceeding with caution

Input sequences must be unaligned and ungapped!
Sequence loaded successfully.
C:\Users\K1807836\Downloads\Ecoli k-12 mg1655 5792691 base pairs.
Sequence loaded successfully.
C:\Users\K1807836\Downloads\Ecoli k-12 mg1655 Boston.gb 4643559 base pairs.
Using weight 15 mers for initial seeds
Creating sorted mer list
Crap.  It's broke, return value 1
Error creating sorted mer list
Exited with error code: -1073741819

Help, please! What does this mean?

[Pierre Lindenbaum]

Hi and welcome. of course it's about "bioinformatics", please change the title and the tags to something meaningful related to your problem.

[Pierre Lindenbaum]

see also: Mauvealigner Error and https://sourceforge.net/p/mauve/mailman/mauve-users/thread/CAK5g6T0Mxe-f0g2ZVjKmPWjw7Xi9nX%3DNtM%2BZSJzw6cKEVsqKmA%40mail.gmail.com/#msg31151554

[GenoMax]

It is safer not to use spaces in filenames. Convert Ecoli k-12 mg1655 to Ecoli_k-12_mg1655.