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Some of these programs have a minimum size threshold for a read to be retained. You could play around with that to keep all trimmed reads 5…
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I'm very new myself and there's plenty of people who can answer your question better than I can, but here is a resource I found extremely h…
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If the number of contigs was so small I would manually check each alignment and edit the header of each contig,inserting the corresponding …
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No the library sizes across conditions should be relatively homogenous because it's from the same dataset. I clustered the samples into sub…
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Thank you for your answer!
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Several hundred to 10000 DEGs.
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Not sure if it is the easiest, but you can map your new assembly to i.e. Dmel genome using LAST:
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