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Recent Replies
Comment: The GDC Legacy Archive is retiring soon.
by
Zhenyu Zhang
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If you are talking about methylation idat files, they were all migrated from legacy to the data portal.
If you are talking about other pl…
Comment: How to access TCGA samples that were treated with a specific drug?
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Zhenyu Zhang
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These TCGA samples were taken 10 ~ 20 years ago, and there is no way they have been treated by the modern immunotherapy.
Are you sure tha…
Comment: Design matrix Differential expression analysis
by
Gordon Smyth
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> When is it recommended to use the design <- cbind(Intercept=1,Group=c(-1,-1,-1,1,1,1))
It is not recommended. No such code appears anywh…
Comment: GRCh38.gmap file
by
lorena9132
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Hi Mathew,
The .txt files provided in the link differ from the .gmap files, but after several attempts, I succeeded in generating the .gma…
Answer: GRCh38.gmap file
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Mathew
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Hi,
I have spent some time searching for a GRCH38 map file for you. I have found a certain publication that uses a hg38 genetic map file…
Answer: Association study using SNP data
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Mathew
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Hi,
You asked three questions so I will attempt to help the best I can
**What should be the optimal GC score cutoff**
It sounds li…
Comment: Design matrix Differential expression analysis
by
SHN
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Thank you for your explanation. I am working on the cell lines and I am investigating the effect of the treatment on the expression level, …
Answer: Issues with Mixture file when using CIBERSORTx
by
vjanve
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I ran into same issue of "duplicated gene symbol(s)":
1: In CIBERSORTxFractions(sigmatrix = sigmatrix, mixture = mixture, :
22292 dup…
Comment: creating batch colum for batch correction
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Expert
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Thank you for your response. These samples were collected from the same patient at different time points(day 0, 8 and 18) and these are ind…
Comment: creating batch colum for batch correction
by
ATpoint
82k
Not sure I follow. Can you first of all describe which comparisons you want to make, pretending there was no batch effect at all. Write dow…
Comment: Segmentation error while using a tool
by
Ruqaiya
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Thank you for the advice, i'll keep that in mind.
Comment: Segmentation error while using a tool
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Ruqaiya
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I am trying to reproduce some bioinformatics data from a research paper-[this paper][1] as i was asked to work on an MRSA bioinfo project, …
Answer: Segmentation error while using a tool
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Mensur Dlakic
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Most likely you didn't do anything wrong. Going by your previous post that you have only 8 GB of RAM, the most likely reason is the lack of…
Comment: Segmentation error while using a tool
by
Thanujay S
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Hey @Ruqaiya!
Can you please give more details on why smalt is opted here?
According to what I can see, the tool has become obsolete! Why …
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