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260
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Comment: Comment: What is the relationship between Illumina P5 P7, universal adapters, and TruSeq
15 days ago by octpus616 ▴ 100
2
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2
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260
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What is the relationship between Illumina P5 P7, universal adapters, and TruSeq Adapter Index 7?
fastqc NGS
15 days ago by octpus616 ▴ 100
1
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1
reply
207
views
How to Annotating Human Genomic Variants for Disease Associations?
R variant-annotation GWAS
updated 11 weeks ago by 43k • written 11 weeks ago by ▴ 100
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0
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381
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Comment: Comment: ATCC cell line source
4 months ago by octpus616 ▴ 100
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2
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381
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ATCC cell line source
cell-line NGS ATCC
4 months ago by octpus616 ▴ 100
1
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1
reply
737
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Comment: Comment: How the first sequencing template removed in pair end sequencing
6 months ago by octpus616 ▴ 100
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0
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737
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Comment: Comment: How the first sequencing product removed in pair end sequencing
6 months ago by octpus616 ▴ 100
5
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7
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737
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How the first sequencing template removed in pair end sequencing
NGS RNA-seq WGS sequence
updated 6 months ago by 141k • written 6 months ago by ▴ 100
0
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0
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271
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how simply mutation annotation in R?
annotation RNA-seq Bioconductor
7 months ago by octpus616 ▴ 100
0
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0
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747
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Comment: Comment: STAR mapping two pass mode for multiple samples with split steps or one step ?
7 months ago by octpus616 ▴ 100
0
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2
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747
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STAR mapping two pass mode for multiple samples with split steps or one step ?
STAR RNA-seq
7 months ago by octpus616 ▴ 100
0
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0
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910
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Comment: Comment: Best practices scRNA-seq guide for beginer
7 months ago by octpus616 ▴ 100
0
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0
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910
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Comment: Comment: Best practices scRNA-seq guide for beginer
7 months ago by octpus616 ▴ 100
0
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1
reply
910
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Comment: Comment: Best practices scRNA-seq guide for beginer
7 months ago by octpus616 ▴ 100
7
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6
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910
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Best practices scRNA-seq guide for beginer
scRNA-seq eBook
7 months ago by octpus616 ▴ 100
0
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0
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318
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Make a variation record dict from vcf by pysam quickly
pysam python vcf
8 months ago by octpus616 ▴ 100
0
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0
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718
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Comment: Comment: How to convert dnsnp in UCSC to gtf
8 months ago by octpus616 ▴ 100
2
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4
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718
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How to convert dnsnp in UCSC to gtf
UCSC dnsnp gtf
8 months ago by octpus616 ▴ 100
0
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0
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718
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Comment: Comment: How to convert dnsnp in UCSC to gtf
8 months ago by octpus616 ▴ 100
2
votes
1
reply
517
views
bedtools random without strand and no overlap
bedtools genome bed
updated 10 months ago by 81k • written 10 months ago by ▴ 100
0
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0
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537
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Comment: Comment: Why is NA18498 sample missing from 1000 genome GRCh38 vcf file?
11 months ago by octpus616 ▴ 100
1
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2
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537
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Why is NA18498 sample missing from 1000 genome GRCh38 vcf file?
NGS vcf 1000 1kg genome
11 months ago by octpus616 ▴ 100
0
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0
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357
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rtracklayer import.chain not accpect chain file
R rtracklayer liftOver
11 months ago by octpus616 ▴ 100
6
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1
reply
465
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what is IGV visualization lighter color variants stand for
visualization vcf IGV
updated 12 months ago by 14k • written 12 months ago by ▴ 100
0
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0
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923
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Comment: Comment: Calculation method of RMSMappingQuality
13 months ago by octpus616 ▴ 100
0
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2
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923
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Calculation method of RMSMappingQuality
NGS bam samtools vcf
13 months ago by octpus616 ▴ 100
0
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0
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347
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How to shift the position of multiallelic variants for phasing analysis with SHAPEIT2?
vcf bcftools shapeit2 phase NGS
updated 10 months ago by 43k • written 13 months ago by ▴ 100
0
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0
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340
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Get RMS MQ for specific POS
vcf bam MQ
13 months ago by octpus616 ▴ 100
2
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1
reply
664
views
bcftools merge without multiallelic sites
NGS bcftools vcf
updated 13 months ago by ▴ 520 • written 13 months ago by ▴ 100
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0
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1.6k
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Comment: Comment: Does running samtools sort in parallel result in unexpected output due to tempor
13 months ago by octpus616 ▴ 100
0
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0
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1.6k
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Comment: Comment: Does running samtools sort in parallel result in unexpected output due to tempor
13 months ago by octpus616 ▴ 100
0
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0
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1.6k
views
Comment: Comment: Does running samtools sort in parallel result in unexpected output due to tempor
13 months ago by octpus616 ▴ 100
3
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7
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1.6k
views
Does running samtools sort in parallel result in unexpected output due to temporary file conflicts?
NGS samtools bam
updated 10 months ago by 43k • written 13 months ago by ▴ 100
0
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0
replies
488
views
Annotate a series of genomic intervals using R as detailed as possible
bed bioconductor annotation R
14 months ago by octpus616 ▴ 100
0
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0
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416
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What is the different bewteen ucsc simple repeat and tandem repeat
reference USCS annotation hg38
14 months ago by octpus616 ▴ 100
0
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0
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1.4k
views
Comment: Comment: samtools calmd and original base quality
14 months ago by octpus616 ▴ 100
2
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2
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1.4k
views
samtools calmd and original base quality
NGS bam samtools
14 months ago by octpus616 ▴ 100
0
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0
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935
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Comment: Comment: GATK haplotypeCaller output MQ in vcf for non variant sites
15 months ago by octpus616 ▴ 100
0
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2
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935
views
GATK haplotypeCaller output MQ in vcf for non variant sites
vcf MQ GATK NGS
15 months ago by octpus616 ▴ 100
0
votes
1
reply
1.2k
views
Comment: Comment: Get length of the alignment from bam file with shell
15 months ago by octpus616 ▴ 100
1
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2
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1.2k
views
Comment: Comment: Get length of the alignment from bam file with shell
15 months ago by octpus616 ▴ 100
1
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6
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1.2k
views
Get length of the alignment from bam file with shell
NGS sam pysam samtools bam
updated 15 months ago by ★ 3.8k • written 15 months ago by ▴ 100
0
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1
reply
1.7k
views
Comment: Comment: Samtools view -e not work
16 months ago by octpus616 ▴ 100
0
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0
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1.7k
views
Comment: Comment: Samtools view -e not work
16 months ago by octpus616 ▴ 100
0
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1
reply
1.7k
views
Comment: Comment: Samtools view -e not work
16 months ago by octpus616 ▴ 100
0
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7
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1.7k
views
Samtools view -e not work
NGS bam sam samtools
updated 15 months ago by 43k • written 16 months ago by ▴ 100
0
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0
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1.7k
views
Comment: Comment: How to determine the encoding method of quality score in a fastaq file?
16 months ago by octpus616 ▴ 100
0
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0
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1.7k
views
Comment: Comment: How to determine the encoding method of quality score in a fastaq file?
16 months ago by octpus616 ▴ 100
4
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6
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1.7k
views
How to determine the encoding method of quality score in a fastaq file?
fastq NGS samtools bwa
16 months ago by octpus616 ▴ 100
0
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0
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1.7k
views
Comment: Comment: How to determine the encoding method of quality score in a fastaq file?
16 months ago by octpus616 ▴ 100
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