I'm running a study on predicting ncRNA's in a bacterium and I used a public rna-seq data (with tree biological replicates) to see if my predicted rna's are being expressed. I got some very good results, however I'm afraid that in order to publish, a qPCR analysis or nothern blot will be required. The problem is since I used public data I don't have access to biological material to do such analysis, how should I proceed? it's seems that almost alI journals require some kind of "experimental" validation, is there some ("good ones" IF > 1.5 maybe?) that do not?
Also, can someone explain me why rna-seq data is not enough to prove that a gene is being expressed?
Thanks in advance.