Hi to all, I have 454 RNA transcripts assembled into Isotigs using Newbler. They are complete, they miss 5' end, they miss 3' end and sometimes they miss both (as revealed by blastx). I want only the CDS (coding sequence) because I want to check the propensity of GC (only in CDS) & hydrophilic amino acids (after translation) in my transcriptome. So do you guys know any tool which does this? So all kind of suggestions are welcome!
thank u raghul