For the fungus I study, transcriptome data were submitted to NCBI by other researchers. Now I want to investigate if some genes are expressed or not, therefore, do you know how to blast against the transcriptome data?
I know that we can blast against a genome online in the NCBI website (http://blast.ncbi.nlm.nih.gov/Blast.cgi?CMD=Web&PAGE_TYPE=BlastHome), but if we can blast against a transcriptome in a similar way? If no, how to download the transcriptome data in order to perform local blast. This is the information I get from the literature: "The RNA-seq expression dataset is available at the NCBI’s Gene Expression Omnibus under the accession code GSE28001."
Thanks in advance for any explanations.