NovaSeq 6000 - 6 Tb data/20 billion reads in 2 days (max possible output)
NovaSeq 5000 - 2 Tb data/1.6 billion reads in 2.5 days
- Four new flowcell types (S1,S2,S3,S4), 2 x 150 bp max, 2 x FC per sequencer.
- On board cluster generation.
- Two reagent cartridges. One for the cluster generation, the other for SBS reagents.
- New type of base call files (*.cbcl), can be converted to fastq.
- $850K for NovaSeq 5000 and $985K for NovaSeq 6000 (from Genohub blog)
A blog entry from Genohub indicates that these sequencers are using TWO color chemistry.
NOTE 1: Two color chemistry has been confirmed.
NOTE 2: Flowcell types can be mixed and matched. S1/S2 are restricted for NovaSeq 5000, all four can be used on NovaSeq 6000. Even though the flowcells have "lanes" like the NextSeq, they may have a single inlet port so only one sample pool per FC. Needs to be confirmed.
PS: If you were hoping to buy one be ready to wait. Apparently Illumina has pre-sold all NovaSeq they can make for a quarter or two this year.
S4 flowcells are now available. 48 human genomes per run (varbatim quote from Illumina). 6 Tb, 20 B reads, 2 x 150 bp.
There is also NovaSeq Xp workflow which allows loading of different pools in each lane. This would be essential for core facilities.
S1,S2 - Flowcells for counting applications
For S2 - PF 1775-2070 cluster/mm^2
S3,S4 - High coverage applications
Same cartridges on both sides (three total).
Reagent Cartridge - Will take one library tube (200-300 pM) per FC.
SBS Reagents - Frozen
SBS Buffers - Room temperature ship/store. Ready to use.
FC looks huge, like NextSeq. Easy to place. No futzing needed.
Run setup identical to current HiSeq sequencers.
Two sides of the instrument can work independently (stream data to BaseSpace on on side, store locally on other side).
Automatic post-run wash once sequencing is complete
Run times includes cluster generation.
Network speed (200 mbps)
ONE library pool per flowcell. Lanes visually distinct but fluidically same
Both 5000/6000 are dual FC systems. For 5000 systems run times slightly longer
Custom primers possible
Asymmetric runs possible (e.g. 100 x 75)
No "Rapid" run capability needed (Illumina's take)
FC's can be used for single and paired-end recipes
ExAMP, all self contained in the sequencer
200/240V, adequate HVAC
Test NovaSeq data is available on BaseSpace.