For Illumina sequencing data with dual indexes (151 read1 + 8 index1 + 8 index2 + 151 read2), conventional demultiplexing method is to set both index1 and index2 for each sample.
However, for some data (i.e. UMI in index2), only index1 is fixed, and index2 is
random. So there is no way to set both index1 and index2 in the sample sheet.
For such case, is it applicable to set only index1 to demultiplex data? Seems bcl2fastq doesn't support such settings. Does any have any experience?