I asked a question regarding how to simulate RNA-seq data online because I can't download big fasta files to run Polyester package on my laptop. But I haven't received a response yet. Now I ask my question differently. Since I can't download the fasta files, if I obtain raw read counts matrix using Galaxy, Can I then simulate this matrix on my laptop? Is it possible and correct to simulate data from raw read counts matrix? I need to simulate some time course read counts to compare some statistical methods for detection of DE genes.