I'm a relative newcomer to RNA-seq analysis, and I am now at the point where I want to do GO term analysis on my dataset.
I have done hierarchical clustering of my dataset consisting of 800 differentially expressed genes from Zebrafish samples. I have identified clusters that are interesting based on their expression patterns, and I now want to look at the gene ontology within these clusters.
I have looked at several R packages for GO term analysis online including clusterProfiler and GOexpress. But the documentation leaves something to be desired for an R newbie like myself. Does anyone have a suggestion for a GO term analysis pipeline they have used in R? Thank you very much!
Best David Tatarakis