I am interested in the recently published SPLiT-Seq single cell/nucleus RNA-Seq paper from Rosenberg et al that came out in Science http://science.sciencemag.org/content/early/2018/03/14/science.aam8999
In this paper the authors describe a custom iterative barcoding strategy to index single cells for single cell RNA-Seq without droplets or cell isolation. They have archived their data on GEO: GSE110823. However I was unable to find any scripts or bioinformatics tools that they used to process the raw data.
I have attempted to throw together a SPLiT-Seq demultiplexing strategy using bash. With the goal of making it more robust, accurate, and functional I wanted to share it with the bioinformatics community. Any comments, criticisms, bug reports, or pointers to any official SPLiT-Seq tools are much appreciated.
Here is a link to the Github repository. https://github.com/paulranum11/splitseq_demultiplexing