I am doing an RNAseq analysis. (using edgeR) I am loading my data for differential analysis, my data is of normalized gene expression(FPKM). I am now just trying to make DEGList object but, it is showing "Negative counts not allowed"
.
I have removed all negative values from the data, it is still showing the error(( Negative counts not allowed).
Please suggest what should i do. thank you!!
Command:
dgeFull <- DGEList(y, group = sampleInfo$condition)
Error: Negative counts not allowed