Hi there, Apologies if this question is a repeat, but I couldn't find the answer I'm looking for on here or youtube. Perhaps someone could point me in the right direction?
I have the DGE RNA-seq data for a gene knock down experiment versus a control (with 3 repeats and 3 control repeats). I have 718 genes and have the log2 fold change (LFC) for all of the genes.
In my .txt file that I read into R: I have gene names in the row, and LFC in a single column. Can I plot a heatmap from this data? I want to be able to visualise which genes are up-regulated or down-regulated and how they cluster. All the other heatmap tutorials I have seen have plotted data for 2 or more columns.
I am unsure how to proceed now I have read this file in.