I having a problem with analyzing my data.. I have 5 time points of 5 patients, So the problem is, when i take all timepoints 0 from all patients and compare them with timepoints 1 from all patients, my p value adj failed. P.s i cant compare each person separately, because i just have one sample per each timepoint, So i at least need two replicate of each sample to do DEG.
Any suggestions are appropriated. Thanks.