Hi, I have to analyze published scRNA-Seq data. Data are Chromium 10X on NovaSeq6000 and: for each sample I have forward (R1) in lane1 and lane2 (and hence two files) and reverse (R2) in lane1 and lane2 (and hence two files). Totally I have 4 files for sample. I have .fastq files. I usually analyze 10X scRNA-Seq data using Cellranger in single strand starting from the demultiplexing and ending with the alignment. It is the first time I have forward and reverse fastq files on multiple lanes and I don't know how to analyse them. Can anyone give me some hints on the analysis I have to do? Suggestions on tutorials, tools, papers about my task are more than welcome.
Thank you in advance