I'm a bioinformatician with lots of NGS experience, but mostly with RNAseq and exomes. I'm looking at an upcoming project which involves the assembly of one or more human genomes. I have no experience in assembly, so what would people suggest I try?
I realise de novo assembly is non-trivial with such a large genome, but what about a reference guided assembly? Can the typical tools help with that, i.e. abyss, velvet or cortex?
Also, what kind of hardware requirements would I need? The biggest box I have access to has 24 cores and 128GB RAM.
Any suggestions gratefully received. Cheers,