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how to split multi-fasta file into single fasta file named by header
Comment: Trying to use hmmer to search genes over metagenome assembled genomes
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Comment: multiqc: group samples by population + boxplot ?
Answer: Trying to use hmmer to search genes over metagenome assembled genomes
Answer: How to determine alternative splicing read counts
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Answer: How do I extract references from ClinVar for all variants in individual gene?
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Pierre Lindenbaum
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get a bed file for your genes and use tabix: tabix --regions genes.bed "https://ftp.ncbi.nlm.nih.gov/pub/clinvar/vcf_GRCh37/clinvar…
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https://www.biostars.org/p/76716/ ; https://www.biostars.org/p/9461227/ ; https://www.biostars.org/p/105388/; https://www.biostars.org/p/38…
Comment: Trying to use hmmer to search genes over metagenome assembled genomes
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Mensur Dlakic
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It is nice that you posted this to give this question a closure, but this is far from an optimal procedure. `esl-translate` is also not a g…
Answer: Trying to use hmmer to search genes over metagenome assembled genomes
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v.berriosfarias
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I finally solved this with the help of the hmmer devs https://github.com/EddyRivasLab/hmmer/issues/302#issuecomment-1539968198 Firstly yo…
Comment: multiqc: group samples by population + boxplot ?
by
Phil Ewels
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Yes the documentation for Custom Content is the worst out of all MultiQC docs. I've been intending to rewrite it for a while now. Best bet …
Comment: Is there a file format better suited for the era of pangenomics than the .vcf? W
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> "Explicit representation: VCF is very explicit about the exact type > and sequence of variation" no, tinyliar, it isn't - ambiguous repr…
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Maxine
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sounds great! waiting for your findings~
Comment: Why some insertions are symbolic in ALT column?
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Maxine
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thank you for sharing your experience!
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Medhat
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There is already an article about the topic that was recently published (I was one of the individuals approached to contribute to this arti…
Comment: Is there a file format better suited for the era of pangenomics than the .vcf? W
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Vincent Laufer
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Apologies - my email address and twitter are listed on my profile page.
Comment: multiqc: group samples by population + boxplot ?
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Pierre Lindenbaum
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It worked by parsing a first run of multiqc, extract the data from the sanitised data , create a SVG plot with R, include the SVG into a fi…
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Ahmed
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thanks, bro I get the solve of my problem by installing new environment and reinstalling samtools software again.
Comment: Solving environment: failed with initial frozen solve. Retrying with flexible so
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Ahmed
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Thanks for telling me that, and i apologize for bad explanation because I am beginner user with the site!
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Pierre Lindenbaum
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what is the output of the following commands: ls -lah '/home/bvs/neelam/AMRFINDER_hypo/hypothetical_analysis/P_aeruginosa_RP73.fasta' …
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GenoMax
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> Cannot open file > '/home/bvs/neelam/AMRFINDER_hypo/hypothetical_analysis/P_aeruginosa_RP73.fasta' Looks like either the file path is in…
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