updated 19 days ago by
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Recent Replies
Comment: Single-cell RNA-seq FindMarker and EnhancedVolcano Plot: most genes are low -log
by
yau
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Dear Mensur,
Thanks so much for your reply!!
In my case, I got 1 control(healthy) and 2 disease mouse Single cell RNA-sew data, so I …
Comment: BWA alignment
by
ATpoint
82k
The relevant code line is precisely the same as in the question.
Answer: imputation through beagle
by
dthorbur
★
1.9k
Building a reference panel from the samples you intend to impute seems like a very circular approach. Plus, GBS is a reduced representation…
Answer: Single-cell RNA-seq FindMarker and EnhancedVolcano Plot: most genes are low -log
by
Mensur Dlakic
★
27k
You seem to have too stringent of a p-value cutoff (1e-5). Hopefully you get the concept of how the interplay of `-log10P-value` and `log2F…
Answer: Single-cell RNA-seq FindMarker and EnhancedVolcano Plot: most genes are low -log
by
yau
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0
![enter image description here][1]
[1]: /media/images/7199a665-b13a-40c8-a065-313cf2b7
Answer: Using samtools with GCS (google cloud storage) on a docker container seems to gi
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aw7
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This is a bit late, but the first thing to go is to run `samtools --version` and make sure the docker samtools is configured with the the G…
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aw7
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I do not think `samtools fixmate` and `markdup` are going to work on PacBio long reads. `fixmate` is for setting and repairing mate inform…
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Azra
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I see, thank you!
Comment: Publish a Letter in higher impact vs Research article in lower impact
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shenwei356
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From the perspective of a Chinese, I'd like to choose a letter in a famous journal with a high IF.
If I'm a PI, I think they are both OK, I…
Comment: Publish a Letter in higher impact vs Research article in lower impact
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ATpoint
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This is off-topic here. I suggest https://academia.stackexchange.com/
Anyway, since hiring procedures in academia are much focused on IF a…
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Vahid
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Thank you very much for your assistance. The problem has been solved; it was an index issue.
Answer: Manual Assembly and Protein Translation, HELP, assignment revision
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Philipp Bayer
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You asked the same question before, and I told you about the reverse complementing (https://www.biostars.org/p/9594474/#9594475 ), and now …
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Zeng Jingyu
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Hi Nikki,
I have to say that I didn't fully understand your question. You said "don't care the association between Instrumental Variable a…
Comment: RNAseq one control two conditions, shared and exclusive genes
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Mohamed Abderrahmane
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I'm not entirely certain about the difference, but I assume it yields similar results. The method you're currently employing seems faster a…
Answer: BWA alignment
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a.alnawfal.1992
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The error [main_samview] suggests that Samtools is expecting a SAM file header but isn't getting one. This often occurs when the pipe (|) b…
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