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3
votes
7
replies
2.1k
views
How much adapters is it ok to keep in my samples after RNA seq?
RNA-seq illumina adapters
updated 17 months ago by 45k • written 22 months ago by ▴ 160
0
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0
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1.6k
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Comment: Comment: Why am I getting different results with kallisto?
22 months ago by bioinfo ▴ 160
0
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1
reply
1.6k
views
Comment: Comment: Why am I getting different results with kallisto?
22 months ago by bioinfo ▴ 160
0
votes
1
reply
1.6k
views
Comment: Comment: Why am I getting different results with kallisto?
22 months ago by bioinfo ▴ 160
3
votes
4
replies
1.6k
views
Why am I getting different results with kallisto?
kallisto
22 months ago by bioinfo ▴ 160
0
votes
1
reply
1.3k
views
Comment: Comment: Are truseq adapter sequences picked up by FASTQC?
22 months ago by bioinfo ▴ 160
2
votes
3
replies
1.3k
views
Are truseq adapter sequences picked up by FASTQC?
fastqc truseq
updated 22 months ago by 153k • written 22 months ago by ▴ 160
0
votes
0
replies
790
views
How to convert dataframe properly to anndata?
single-cell python anndata
updated 17 months ago by 45k • written 23 months ago by ▴ 160
0
votes
0
replies
751
views
How to import filtered_tf_bc_matrix and add it to my anndata object?
python anndata atac-seq
updated 17 months ago by 45k • written 23 months ago by ▴ 160
0
votes
0
replies
641
views
How to subsample ATAC fragment file?
single-cell atac-seq scRNA-seq
updated 17 months ago by 45k • written 23 months ago by ▴ 160
0
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0
replies
1.8k
views
Comment: Comment: How to assign gene names after kallisto when I add GFP?
23 months ago by bioinfo ▴ 160
0
votes
1
reply
1.8k
views
Comment: Comment: How to assign gene names after kallisto when I add GFP?
23 months ago by bioinfo ▴ 160
0
votes
2
replies
3.8k
views
Removing constant/zero columns for PCA?
R pca
updated 23 months ago by ★ 30k • written 23 months ago by ▴ 160
1
vote
4
replies
1.8k
views
How to assign gene names after kallisto when I add GFP?
tximport RNA-seq kallisto
23 months ago by bioinfo ▴ 160
0
votes
0
replies
5.9k
views
Comment: Comment: How to plot proportion of cells in each cluster with scanpy?
24 months ago by bioinfo ▴ 160
0
votes
0
replies
5.9k
views
Comment: Comment: How to plot proportion of cells in each cluster with scanpy?
24 months ago by bioinfo ▴ 160
1
vote
8
replies
5.9k
views
How to plot proportion of cells in each cluster with scanpy?
scRNA-seq scanpy single-cell
24 months ago by bioinfo ▴ 160
0
votes
1
reply
5.9k
views
Comment: Comment: How to plot proportion of cells in each cluster with scanpy?
24 months ago by bioinfo ▴ 160
0
votes
1
reply
5.9k
views
Comment: Comment: How to plot proportion of cells in each cluster with scanpy?
24 months ago by bioinfo ▴ 160
0
votes
0
replies
1.3k
views
Comment: Comment: How to calculate GC content of reads that mapped to a specific gene?
2.0 years ago by bioinfo ▴ 160
5
votes
2
replies
1.3k
views
How to calculate GC content of reads that mapped to a specific gene?
STAR RNA-seq
2.0 years ago by bioinfo ▴ 160
0
votes
0
replies
1.8k
views
Comment: Comment: Can the DESeq2 object for paired data be set with design= ~ batch + condition?
2.1 years ago by bioinfo ▴ 160
0
votes
1
reply
1.8k
views
Comment: Comment: Can the DESeq2 object for paired data be set with design= ~ batch + condition?
2.1 years ago by bioinfo ▴ 160
0
votes
0
replies
1.8k
views
Comment: Comment: Can the DESeq2 object for paired data be set with design= ~ batch + condition?
2.1 years ago by bioinfo ▴ 160
0
votes
1
reply
1.8k
views
Comment: Comment: Can the DESeq2 object for paired data be set with design= ~ batch + condition?
2.1 years ago by bioinfo ▴ 160
0
votes
8
replies
1.8k
views
Can the DESeq2 object for paired data be set with design= ~ batch + condition?
rna-seq deseq2
2.1 years ago by bioinfo ▴ 160
1
vote
1
reply
1.4k
views
Do I need to perform basic filtering before using scrublet?
scanpy scrublet
updated 2.1 years ago by 153k • written 2.1 years ago by ▴ 160
0
votes
0
replies
2.4k
views
Comment: Comment: Questions about per base sequence quality and GC content.
2.1 years ago by bioinfo ▴ 160
0
votes
1
reply
2.4k
views
Comment: Comment: Questions about per base sequence quality and GC content.
2.1 years ago by bioinfo ▴ 160
0
votes
0
replies
2.7k
views
Comment: Comment: How to choose threshold for scrublet?
2.1 years ago by bioinfo ▴ 160
0
votes
2
replies
2.7k
views
How to choose threshold for scrublet?
rna-seq scrublet
2.1 years ago by bioinfo ▴ 160
0
votes
0
replies
2.4k
views
Comment: Comment: Questions about per base sequence quality and GC content.
2.1 years ago by bioinfo ▴ 160
1
vote
5
replies
2.4k
views
Questions about per base sequence quality and GC content.
fastqc fastq seq RNA
2.1 years ago by bioinfo ▴ 160
0
votes
0
replies
1.4k
views
Comment: Comment: How to assign gene names to ensembl IDs if species not in Biomart
2.1 years ago by bioinfo ▴ 160
3
votes
3
replies
1.4k
views
How to assign gene names to ensembl IDs if species not in Biomart
biomart ensembl
2.1 years ago by bioinfo ▴ 160
0
votes
2
replies
5.0k
views
Comment: Comment: Why do I need to sort and index gtf file for IGV?
2.2 years ago by bioinfo ▴ 160
0
votes
4
replies
5.0k
views
Why do I need to sort and index gtf file for IGV?
igv
updated 2.2 years ago by 89k • written 2.2 years ago by ▴ 160
0
votes
0
replies
1.3k
views
Comment: Comment: Where can I find the --chromosomes file for kallisto?
2.2 years ago by bioinfo ▴ 160
3
votes
3
replies
1.3k
views
Where can I find the --chromosomes file for kallisto?
kallisto
updated 2.2 years ago by 153k • written 2.2 years ago by ▴ 160
3
votes
2
replies
1.3k
views
How to figure out adapter sequences in RNA seq?
adapters rna-seq
updated 2.2 years ago by ★ 1.6k • written 2.2 years ago by ▴ 160
1
vote
0
replies
693
views
How does kallisto genomebam handle multi mapping reads?
kallisto
updated 2.3 years ago by 45k • written 2.3 years ago by ▴ 160
0
votes
0
replies
1.5k
views
Comment: Comment: Does adding reads cause batch effects?
2.3 years ago by bioinfo ▴ 160
0
votes
1
reply
1.5k
views
Comment: Comment: Does adding reads cause batch effects?
2.3 years ago by bioinfo ▴ 160
4
votes
5
replies
1.5k
views
Does adding reads cause batch effects?
kallisto RNAseq
2.3 years ago by bioinfo ▴ 160
0
votes
0
replies
871
views
Do I need to do QC before demultiplexing hashtags with hashsolo?
scanpy hashtag hashsolo RNAseq
2.4 years ago by bioinfo ▴ 160
1
vote
0
replies
2.5k
views
Comment: Comment: How to demultiplex data with totalseq B antibodies using hashsolo?
2.4 years ago by bioinfo ▴ 160
0
votes
1
reply
2.5k
views
Comment: Comment: How to demultiplex data with totalseq B antibodies using hashsolo?
2.4 years ago by bioinfo ▴ 160
1
vote
3
replies
2.5k
views
How to demultiplex data with totalseq B antibodies using hashsolo?
cellranger. seq hashsolo rna
2.4 years ago by bioinfo ▴ 160
0
votes
0
replies
1.1k
views
Comment: Comment: Do I need to specify the introns on the gtf file for cellranger?
2.4 years ago by bioinfo ▴ 160
0
votes
2
replies
1.1k
views
Do I need to specify the introns on the gtf file for cellranger?
fasta gtf cellranger scRNAseq
updated 2.4 years ago by 153k • written 2.4 years ago by ▴ 160
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