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236
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On applying spike-in-derived scaling factors to ChIP-seq data: adjust for background signal (input) or not?
spike-in
deepTools
normalization
ChIP-seq
scaling-factor
5 days ago by
kalavattam
▴ 280
1
vote
1
reply
1.2k
views
Comment:
Comment: Is a SLURM job array submission suitable for parallelizing multiple FASTQ file d
5 weeks ago by
kalavattam
▴ 280
9
votes
12
replies
1.2k
views
Is a SLURM job array submission suitable for parallelizing multiple FASTQ file downloads?
parallel-computing
HPC
SLURM
FASTQ
updated 4 weeks ago by
Dave Carlson
★ 2.1k • written 5 weeks ago by
kalavattam
▴ 280
0
votes
0
replies
353
views
Comment:
Comment: Bos Taurus, Bos Indicus and Bubalus bubalis GTF,GFF,GFF3 file
9 weeks ago by
kalavattam
▴ 280
0
votes
0
replies
353
views
Comment:
Comment: Bos Taurus, Bos Indicus and Bubalus bubalis GTF,GFF,GFF3 file
9 weeks ago by
kalavattam
▴ 280
0
votes
0
replies
470
views
Answer:
Answer: remove the letter a from a biplot in ggplot
3 months ago by
kalavattam
▴ 280
0
votes
0
replies
352
views
Adjusting MACS3 --slocal and --llocal parameters for small-genome organisms like S. cerevisiae?
yeast
poisson
ChIP-seq
macs3
peak-calling
3 months ago by
kalavattam
▴ 280
1
vote
1
reply
419
views
Seeking advice on methods to calculate ENCODE ChIP-seq data standard M_DISTINCT
ChIP-seq
quality-check
ENCODE
library-complexity
3 months ago by
kalavattam
▴ 280
1
vote
0
replies
419
views
Answer:
Answer: Seeking advice on methods to calculate ENCODE ChIP-seq data standard M_DISTINCT
3 months ago by
kalavattam
▴ 280
0
votes
0
replies
3.4k
views
Comment:
Comment: Seeking feedback on ChIP-seq normalization method: Calculating scaling factors b
4 months ago by
kalavattam
▴ 280
0
votes
0
replies
559
views
Comment:
Comment: Low "assigned alignments" in output and high "Unassigned_NoFeatures" in summary
4 months ago by
kalavattam
▴ 280
2
votes
1
reply
594
views
Comment:
Comment: What should be the term, "insert" or "insert size"?
4 months ago by
kalavattam
▴ 280
1
vote
0
replies
525
views
Answer:
Answer: Does single-end short-read sequencing affect the mapping of mitochondrial and ri
5 months ago by
kalavattam
▴ 280
0
votes
0
replies
1.9k
views
Comment:
Comment: How can I convert multiple bam files in a directory into fastq files using samt
5 months ago by
kalavattam
▴ 280
0
votes
2
replies
1.9k
views
Comment:
Comment: How can I convert multiple bam files in a directory into fastq files using samt
5 months ago by
kalavattam
▴ 280
0
votes
1
reply
1.9k
views
Comment:
Comment: How can I convert multiple bam files in a directory into fastq files using samt
5 months ago by
kalavattam
▴ 280
0
votes
1
reply
1.9k
views
Comment:
Comment: How can I convert multiple bam files in a directory into fastq files using samt
5 months ago by
kalavattam
▴ 280
0
votes
1
reply
1.9k
views
Comment:
Comment: How can I convert multiple bam files in a directory into fastq files using samt
5 months ago by
kalavattam
▴ 280
0
votes
1
reply
1.9k
views
Comment:
Comment: How can I convert multiple bam files in a directory into fastq files using samt
5 months ago by
kalavattam
▴ 280
0
votes
1
reply
1.9k
views
Comment:
Comment: How can I convert multiple bam files in a directory into fastq files using samt
5 months ago by
kalavattam
▴ 280
0
votes
1
reply
1.9k
views
Comment:
Comment: How can I convert multiple bam files in a directory into fastq files using samt
5 months ago by
kalavattam
▴ 280
3
votes
2
replies
1.9k
views
Answer:
Answer: How can I convert multiple bam files in a directory into fastq files using samt
5 months ago by
kalavattam
▴ 280
0
votes
0
replies
3.0k
views
Answer:
Answer: Reheadering a subsetted bam gives truncated file
5 months ago by
kalavattam
▴ 280
1
vote
1
reply
654
views
Comment:
Comment: What is the difference between Enrichr and ShinyGO? Which one is the best?
5 months ago by
kalavattam
▴ 280
1
vote
1
reply
654
views
Comment:
Comment: What is the difference between Enrichr and ShinyGO? Which one is the best?
5 months ago by
kalavattam
▴ 280
1
vote
3
replies
895
views
Clarification regarding SAM flags "mate reverse strand" (flag 16/0x10) and "read reverse strand" (flag 32/0x20)
PE
BAM
SAM
flag
paired-end
7 months ago by
kalavattam
▴ 280
1
vote
0
replies
895
views
Answer:
Answer: Clarification regarding SAM flags "mate reverse strand" (flag 16/0x10) and "read
7 months ago by
kalavattam
▴ 280
0
votes
0
replies
895
views
Comment:
Comment: Clarification regarding SAM flags "mate reverse strand" (flag 16/0x10) and "read
7 months ago by
kalavattam
▴ 280
0
votes
0
replies
316
views
Seeking blacklist (exclusion list) for S. cerevisiae in ChIP-seq analysis
QC
blacklist
ChIP-seq
10 months ago by
kalavattam
▴ 280
2
votes
1
reply
453
views
Optimal MACS2/3 configuration for paired-end ChIP-seq data: `-f BAM` versus `-f BAMPE`
MACS3
peak-calling
MACS2
ChIP-seq
updated 10 months ago by
ATpoint
86k • written 10 months ago by
kalavattam
▴ 280
0
votes
0
replies
19k
views
Comment:
Comment: BEDOPS gtf2bed conversion error with Ensembl GTF
13 months ago by
kalavattam
▴ 280
0
votes
0
replies
1.5k
views
Comment:
Comment: Is there a way to subsample Hi-C at Hi-C matrix .cool file?
14 months ago by
kalavattam
▴ 280
0
votes
1
reply
1.7k
views
Comment:
Comment: How to convert data in r?
14 months ago by
kalavattam
▴ 280
1
vote
1
reply
1.7k
views
Comment:
Comment: How to convert data in r?
14 months ago by
kalavattam
▴ 280
0
votes
0
replies
3.4k
views
Comment:
Comment: Seeking feedback on ChIP-seq normalization method: Calculating scaling factors b
15 months ago by
kalavattam
▴ 280
0
votes
1
reply
3.4k
views
Comment:
Comment: Seeking feedback on ChIP-seq normalization method: Calculating scaling factors b
15 months ago by
kalavattam
▴ 280
0
votes
1
reply
1.5k
views
Answer:
Answer: Is there a way to subsample Hi-C at Hi-C matrix .cool file?
15 months ago by
kalavattam
▴ 280
0
votes
0
replies
2.0k
views
Comment:
Comment: ChIP-seq visualization: Is it valid to do a coverage normalization in addition t
15 months ago by
kalavattam
▴ 280
0
votes
2
replies
2.0k
views
Comment:
Comment: ChIP-seq visualization: Is it valid to do a coverage normalization in addition t
updated 15 months ago by
Ram
44k • written 15 months ago by
kalavattam
▴ 280
0
votes
1
reply
929
views
Comment:
Comment: Centrality analysis to identify TFs
15 months ago by
kalavattam
▴ 280
6
votes
5
replies
2.0k
views
ChIP-seq visualization: Is it valid to do a coverage normalization in addition to applying a spike-in-derived scaling factor?
ChIP-seq
normalization
scaling-factor
coverage
spike-in
updated 15 months ago by
Ram
44k • written 15 months ago by
kalavattam
▴ 280
0
votes
0
replies
1.8k
views
Answer:
Answer: What is an appropriate cut off for p-values and log fold changes during the anal
15 months ago by
kalavattam
▴ 280
0
votes
1
reply
3.4k
views
Comment:
Comment: Seeking feedback on ChIP-seq normalization method: Calculating scaling factors b
15 months ago by
kalavattam
▴ 280
0
votes
0
replies
3.4k
views
Comment:
Comment: Seeking feedback on ChIP-seq normalization method: Calculating scaling factors b
16 months ago by
kalavattam
▴ 280
0
votes
1
reply
3.4k
views
Comment:
Comment: Seeking feedback on ChIP-seq normalization method: Calculating scaling factors b
16 months ago by
kalavattam
▴ 280
0
votes
1
reply
3.4k
views
Comment:
Comment: Seeking feedback on ChIP-seq normalization method: Calculating scaling factors b
15 months ago by
kalavattam
▴ 280
10
votes
13
replies
3.4k
views
Seeking feedback on ChIP-seq normalization method: Calculating scaling factors by dividing input by IP, including spike-in
ChIP-seq
spike-in
scaling-factor
coverage
3 months ago by
kalavattam
▴ 280
1
vote
1
reply
949
views
Spike-in normalization via deepTools multiBamSummary --scalingFactors: What's an appropriate --binSize?
spike-in
ChIP-seq
deepTools
updated 16 months ago by
rfran010
★ 1.3k • written 16 months ago by
kalavattam
▴ 280
0
votes
0
replies
253k
views
Comment:
Comment: Tools To Calculate Average Coverage For A Bam File?
16 months ago by
kalavattam
▴ 280
0
votes
0
replies
253k
views
Comment:
Comment: Tools To Calculate Average Coverage For A Bam File?
16 months ago by
kalavattam
▴ 280
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