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Recent Replies
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C: Cluster sub-set analysis using Seurat by
igor ♦ 11k
Subsetting just keeps/removes specific cells. It does not alter their metadata.
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A: Getting the assembled genome using the BAM file obtained from reference mapping by
h.mon ♦ 30k
To get an assembled genome, you need to assemble the reads, not map them onto a reference genome....
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A: Trimmomatic and Salmon error by
Rob ♦ 4.0k
It looks like you have an inconsistent set of modules loaded on the nodes where younare running? ...
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A: volcano plot help code by
Kevin Blighe ♦ 61k
Did you create this github issue? - https://github.com/kevinblighe/EnhancedVolcano/issues/52 Try...
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C: METAPHLAN: Getting genus level resolution but not family level by
DC7 • 20
I am not getting any script to merge all the metaphlan `.biom` outputs which I should do prior to...
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C: Differential Expression analysis between single cell RNa seq & bulk RNa seq data by
bioyas • 0
As you said it does not make sense to do this analysis without modification.. IF we do DE without...
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C: Cluster sub-set analysis using Seurat by
singcell • 0
Thank you for your reply. I have got integrated Seurat object containing KO and WT samples. Can I...
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A: How to extract reads that map with BWA exactly twice by
thomashartwig80 • 0
Yes, chromosomes have the names of their parents to distinguish. We use a chain file from WGA to ...
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C: Bowtie2 reference genome by
genomax ♦ 85k
It may not since once a read starts multi-mapping it would become subject to limits for those. If...
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C: METAPHLAN: Getting genus level resolution but not family level by
cpad0112 ♦ 13k
did you use biom to sff convert script provided by stamp team?
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C: Softwares for statistitical analysis of MetaPhlAn output by
DC7 • 20
Yes sir. The database is CHOCOPHLAN v30 which is recommended by MetPhlAn3. I have created a threa...
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A: R: poppr: Problems converting vcf to genclone object by
evolozzy • 10
I've found a solution, using `pegas` with`vcfR`. This causes another problem but it is easy to ...
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