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Recent Replies
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Fungal ITS2 region (length is really variable so read-through can be an issue)
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Also try the NCBI AWS bucket transfer tool. It's extremely convenient, however it's not instantaneous and you need to have a (paid) AWS acc…
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Check a random sampling of reads to make sure the adapters are being removed. You may also want to blast a few reads to ensure that there i…
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Many thanks for your comment. If read-through is happening, shouldn't Cutadapt being taking care of that there? I provided to the Cutadapt …
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Thank you. I will read the documentation you attached. Are you going to slowly phase out the not kb ref commands? Also, does the t2g.txt fi…
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> I need to understand why is this happening
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I think this is a question for CLC support. It should be trivial for them to combine these fields into one. In other words, you need a bett…
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Although this probably doesn't qualify as `programmatically retrieving positions`, you can always do it by sequence alignments. This can be…
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The error message points to a problem with `prodigal-gv`. What happens when you type `prodigal-gv` on the command line?
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For anyone it might be useful: a colleague of mine, who was not dumb to use the wrong annotation `gtf` as I did, also observed an important…
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Hello people sorry I am dumb the problem lied in the fact that I had mouse cells but my `.gtf` transcriptome file was for the human. Now wi…
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Can you indicate if you need to do this within CLC or can use an external solution (if someone provides it here). If you need to do this in…
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