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Recent Replies
Comment: scRNA-seq: Consistent low number of cells and low fraction reads across the samp
by
jv
★
1.2k
I wonder if using cell hashing, which would allow pooling of cells from multiple samples before droplet formation, could improve things her…
Comment: Where Can I Find The Basepair Positions Of Chromosome Bands?
by
Malachi Griffith
19k
And the hg38 version can be found here:
https://hgdownload.soe.ucsc.edu/goldenPath/hg38/database/cytoBand.txt.gz
And for convenience, upda…
Answer: How Can I move the scattered dots more closer into the center of box ?
by
Trivas
★
1.5k
You added a position dodge on your boxplot but not on your points.
Comment: How Can I move the scattered dots more closer into the center of box ?
by
ATpoint
78k
Please understand that biostars is not a helpdesk for trivial ggplot questions. Google it and find help on previous StackExchange sites, or…
Comment: Yes .. BBMap can do that! - Part III clumpify (mark (and dedupe) duplicates with
by
jucosie
•
0
Hello,
I am reviewing some old scripts in which I used clumpify.sh (BBMap 38.90) with the following parameters:
./clumpify.sh in1=$dir_c…
Comment: Problematic fastq files...How can we trust them?
by
Istvan Albert
99k
perhaps ask her about the origin of the data, how was the data postprocessed
FASTQ data is a bit more resilient than other types of data a…
Answer: GO categorization
by
Istvan Albert
99k
If you have an organism that lacks the GO to gene mapping then you have to find a way to assign these terms to your genes.
Basically your …
Comment: doubt about usage of megahit
by
Istvan Albert
99k
you have not mentioned what kind of resources you have, how much data you have to process and what the expected genome/transcriptome sizes …
Comment: Problematic fastq files...How can we trust them?
by
ATpoint
78k
Then tell her to bring proper data. In the lab you also don't use buffers with mold growing in it, and in bioinformatics we don't start wit…
Comment: Problematic fastq files...How can we trust them?
by
blackadder
▴
30
Its not my data. An external researcher is visiting and she wanted from us to do some bioinformatics... The samples were not sequenced by u…
Comment: Tools or R / Python packages to perfom ML models
by
sil_bioinfo
▴
30
Oh, I understand. Thank you so much for your help!
The truth is that there is so much information, so many packages, libraries, tutorials,…
Comment: Tools or R / Python packages to perfom ML models
by
Matthias Zepper
4.2k
Just to clarify:
Many ML models are trained and executed using dedicated software libraries for machine learning and artificial intellige…
Answer: Problematic fastq files...How can we trust them?
by
ATpoint
78k
> I assume that maybe all these are fixable with a script but my question is, can we trust these files? Is it worth spending time and effor…
Comment: Tools or R / Python packages to perfom ML models
by
sil_bioinfo
▴
30
My main idea is to be able to classify patients with active TB, LTBI and healthy patients as well as possible. The problem is that within t…
Comment: scRNA-seq: Consistent low number of cells and low fraction reads across the samp
by
rpolicastro
12k
They could have saved themselves a ton of money by sequencing this on a miseq.
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