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0
votes
0
replies
510
views
Tools to model DEG/pathways in (fast-dividing) CANCER cell lines across timepoints? (in bulk RNA-seq)
DEG
RNA-seq
bulk
6 weeks ago by
txema.heredia
▴ 250
0
votes
0
replies
3.4k
views
Answer:
Answer: Draw GO tree for some GO ids with coloring
9 weeks ago by
txema.heredia
▴ 250
0
votes
0
replies
996
views
Comment:
Comment: DESeq2 design for differential expression with 2 timepoints and 2 controls
11 weeks ago by
txema.heredia
▴ 250
0
votes
0
replies
409
views
KEGG pathways. How to plot the log2FC of genes collapsed into a "node"?
visualization
DEG
KEGG
R
pathway
11 weeks ago by
txema.heredia
▴ 250
0
votes
1
reply
761
views
Comment:
Comment: Biological meaning of metrics commonly used for QC in scRNA-seq?
3 months ago by
txema.heredia
▴ 250
8
votes
3
replies
761
views
Biological meaning of metrics commonly used for QC in scRNA-seq?
ribosome
QC
velocity
single-cell
intron
updated 3 months ago by
jared.andrews07
★ 19k • written 3 months ago by
txema.heredia
▴ 250
1
vote
1
reply
1.1k
views
Comment:
Comment: Differeces between Seurat::RunPCA and scater::runPCA affect downstream analyses
4 months ago by
txema.heredia
▴ 250
0
votes
0
replies
13k
views
Comment:
Comment: Seurat3: RNA vs SCT assays for DotPlot
4 months ago by
txema.heredia
▴ 250
1
vote
0
replies
2.9k
views
Comment:
Comment: Single cell demultiplexing using 10x and TotalSeq-B
4 months ago by
txema.heredia
▴ 250
0
votes
1
reply
1.1k
views
Comment:
Comment: Differeces between Seurat::RunPCA and scater::runPCA affect downstream analyses
4 months ago by
txema.heredia
▴ 250
1
vote
5
replies
1.1k
views
Differeces between Seurat::RunPCA and scater::runPCA affect downstream analyses
slingshot
PCA
Seurat
scater
single-cell
updated 4 months ago by
Bastien Hervé
6.5k • written 4 months ago by
txema.heredia
▴ 250
0
votes
0
replies
843
views
Comment:
Comment: Best choices for DGE and pathway enrichment analysis in single cell data using p
4 months ago by
txema.heredia
▴ 250
5
votes
2
replies
843
views
Best choices for DGE and pathway enrichment analysis in single cell data using pseudobulk?
DEG
GSEA
single-cell
pseudobulk
4 months ago by
txema.heredia
▴ 250
0
votes
0
replies
1.3k
views
Comment:
Comment: Tool for automatic immune cell annotation in MOUSE?
5 months ago by
txema.heredia
▴ 250
0
votes
1
reply
1.3k
views
Comment:
Comment: Tool for automatic immune cell annotation in MOUSE?
6 months ago by
txema.heredia
▴ 250
3
votes
5
replies
1.3k
views
Tool for automatic immune cell annotation in MOUSE?
immune
single-cell
annotation
5 months ago by
txema.heredia
▴ 250
0
votes
0
replies
2.3k
views
Comment:
Comment: What is the current state of the beef against UMAP/tSNE in the context of sc tra
6 months ago by
txema.heredia
▴ 250
12
votes
6
replies
2.3k
views
Forum:
What is the current state of the beef against UMAP/tSNE in the context of sc trajectory analysis/monocle3?
monocle3
umap
single-cell
tSNE
trajectory
6 months ago by
txema.heredia
▴ 250
0
votes
1
reply
2.3k
views
Comment:
Comment: What is the current state of the beef against UMAP/tSNE in the context of sc tra
6 months ago by
txema.heredia
▴ 250
0
votes
0
replies
586
views
Comment:
Comment: Detect changes in cell viability with bulk RNA-seq?
7 months ago by
txema.heredia
▴ 250
1
vote
2
replies
586
views
Detect changes in cell viability with bulk RNA-seq?
DEG
viability
RNA
7 months ago by
txema.heredia
▴ 250
0
votes
0
replies
503
views
Minimum number of replicates when doing scRNA-seq using cell-lines?
design
10x
single-cell
10 months ago by
txema.heredia
▴ 250
1
vote
1
reply
1.2k
views
Answer:
Answer: DESeq2: adjusted p-values become NA when sub-setting samples
10 months ago by
txema.heredia
▴ 250
0
votes
0
replies
5.2k
views
Comment:
Comment: collapsePathways function throwing error
10 months ago by
txema.heredia
▴ 250
3
votes
4
replies
1.2k
views
DESeq2: adjusted p-values become NA when sub-setting samples
R
DEG
RNA-seq
DESeq2
updated 10 months ago by
GenoMax
153k • written 11 months ago by
txema.heredia
▴ 250
6
votes
3
replies
1.2k
views
Does single-end short-read sequencing affect the mapping of mitochondrial and ribosomal RNA?
pair-end
single-end
RNA-seq
updated 14 months ago by
kalavattam
▴ 380 • written 14 months ago by
txema.heredia
▴ 250
2
votes
0
replies
1.2k
views
Comment:
Comment: Does single-end short-read sequencing affect the mapping of mitochondrial and ri
14 months ago by
txema.heredia
▴ 250
0
votes
0
replies
739
views
Comment:
Comment: Single cell - integration after demultiplexing samples?
15 months ago by
txema.heredia
▴ 250
0
votes
2
replies
739
views
Single cell - integration after demultiplexing samples?
integration
demultiplexing
single-cell
15 months ago by
txema.heredia
▴ 250
3
votes
4
replies
5.0k
views
Single-cell ambient RNA correction: SoupX vs decontX contamination fraction
single-cell
ambient-RNA
updated 16 months ago by
fracarb8
★ 1.7k • written 16 months ago by
txema.heredia
▴ 250
0
votes
1
reply
5.0k
views
Answer:
Answer: Single-cell ambient RNA correction: SoupX vs decontX contamination fraction
16 months ago by
txema.heredia
▴ 250
0
votes
0
replies
1.8k
views
Comment:
Comment: How to handle duplicated genes in TCGA data?
16 months ago by
txema.heredia
▴ 250
0
votes
2
replies
1.8k
views
Answer:
Answer: How to handle duplicated genes in TCGA data?
16 months ago by
txema.heredia
▴ 250
0
votes
0
replies
5.0k
views
Comment:
Comment: Single-cell ambient RNA correction: SoupX vs decontX contamination fraction
16 months ago by
txema.heredia
▴ 250
0
votes
1
reply
14k
views
Comment:
Comment: biomaRt: Timeout on getBM().
17 months ago by
txema.heredia
▴ 250
1
vote
0
replies
3.1k
views
Answer:
Answer: KEGG analysis in R
17 months ago by
txema.heredia
▴ 250
0
votes
0
replies
485
views
Inferring cell identity/genotype in single cells with missing information
cell
seurat
single
score
17 months ago by
txema.heredia
▴ 250
0
votes
1
reply
2.9k
views
Comment:
Comment: Single cell demultiplexing using 10x and TotalSeq-B
18 months ago by
txema.heredia
▴ 250
1
vote
5
replies
2.9k
views
Single cell demultiplexing using 10x and TotalSeq-B
demultiplexing
cellranger
antibodies
single-cell
4 months ago by
txema.heredia
▴ 250
0
votes
0
replies
1.4k
views
Comment:
Comment: Can I infer the fraction of replicating cells from bulk RNA-seq data?
updated 24 months ago by
Ram
45k • written 24 months ago by
txema.heredia
▴ 250
2
votes
3
replies
1.4k
views
Can I infer the fraction of replicating cells from bulk RNA-seq data?
replication
apoptosis
updated 24 months ago by
Ram
45k • written 24 months ago by
txema.heredia
▴ 250
2
votes
1
reply
1.0k
views
DESeq2 design comparing changes through 2 timepoints of 2 different treatments.
RNA-seq
deseq2
updated 2.0 years ago by
swbarnes2
15k • written 2.0 years ago by
txema.heredia
▴ 250
2
votes
7
replies
2.5k
views
Tool to search for missing genes among genome annotation versions?
annotation
updated 2.1 years ago by
LauferVA
4.8k • written 2.1 years ago by
txema.heredia
▴ 250
0
votes
1
reply
2.5k
views
Comment:
Comment: Tool to search for missing genes among genome annotation versions?
2.1 years ago by
txema.heredia
▴ 250
0
votes
1
reply
1.2k
views
Help manually processing strand in paired-end reads when fishing for lariats in bulk RNA-seq
lariat
splicing
rna-seq
2.2 years ago by
txema.heredia
▴ 250
0
votes
0
replies
1.2k
views
Answer:
Answer: Help manually processing strand in paired-end reads when fishing for lariats in
2.2 years ago by
txema.heredia
▴ 250
0
votes
0
replies
654
views
Differential Gene Expression analysis in heterogeneous tissues
RNA-seq
deseq2
updated 2.3 years ago by
Ram
45k • written 2.3 years ago by
txema.heredia
▴ 250
0
votes
0
replies
5.2k
views
Comment:
Comment: Aberrant splicing in bulk RNAseq
2.4 years ago by
txema.heredia
▴ 250
0
votes
1
reply
5.2k
views
Comment:
Comment: Aberrant splicing in bulk RNAseq
2.4 years ago by
txema.heredia
▴ 250
0
votes
1
reply
5.2k
views
Comment:
Comment: Aberrant splicing in bulk RNAseq
2.4 years ago by
txema.heredia
▴ 250
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