8
votes
16
replies
829
views
4
votes
7
replies
514
views
7 follow
updated 9 hours ago by
dsull
★
5.8k
•
written 4 days ago by
qudrat.nii
▴
10
4
votes
8
replies
453
views
3
votes
0
replies
116
views
updated 18 hours ago by
Ram
43k
•
written 1 day ago by
Claire Watson
▴
60
3
votes
4
replies
328
views
updated 1 hour ago by
BioinfGuru
★
1.7k
•
written 2 days ago by
Ming Tommy Tang
★
3.9k
2
votes
6
replies
1.8k
views
7 follow
1
vote
1
reply
102
views
updated 16 hours ago by
Gordon Smyth
★
7.0k
•
written 18 hours ago by
pairedttest
▴
10
1
vote
1
reply
123
views
1
vote
6
replies
313
views
4 hours ago by
starswillfade
▴
10
0
votes
2
replies
184
views
updated 18 hours ago by
Ram
43k
•
written 1 day ago by
The_PyPanda
▴
10
0
votes
3
replies
238
views
0
votes
1
reply
105
views
updated 11 hours ago by
Gordon Smyth
★
7.0k
•
written 1 day ago by
hagl
▴
10
0
votes
0
replies
88
views
0
votes
0
replies
75
views
0
votes
1
reply
95
views
updated 8 hours ago by
Mensur Dlakic
★
27k
•
written 15 hours ago by
O.rka
▴
710
0
votes
2
replies
131
views
0
votes
7
replies
314
views
0
votes
5
replies
266
views
updated 31 minutes ago by
Istvan Albert
100k
•
written 3 days ago by
sehriban.buyukkilic
▴
10
0
votes
0
replies
90
views
0
votes
0
replies
183
views
Recent Votes
Recent Locations •
All
France, 1 minute ago
Thailand, 2 minutes ago
United States, 8 minutes ago
Brazil, 16 minutes ago
University Park, USA, 21 minutes ago
Australia, 21 minutes ago
Bologna, 22 minutes ago
Recent Awards •
All
Popular Question to
rohitsatyam102
▴
850
Popular Question to
candron
▴
10
Scholar to
Alex Reynolds
35k
Popular Question to
chaco001
▴
40
Commentator to
GenoMax
141k
Popular Question to
rheab1230
▴
140
Popular Question to
BioinfGuru
★
1.7k
Recent Replies
Comment: different FeatureCounts output for the same data
by
Istvan Albert
100k
just to clarify, it is not that the program algorithm works differently but the meaning of the flags changed;
before -p was sufficient to…
Comment: how to combine multiple RNAseq count files into a single dataframe in R and unix
by
BioinfGuru
★
1.7k
Thanks Ram. Using merge maxed out my ram on a large list, but your suggestion directed me to purrr::reduce which works great.
Comment: ScRNA data question
by
starswillfade
▴
10
features <- SelectIntegrationFeatures(object.list = merged_dat)
data.anchors <- FindIntegrationAnchors(object.list = merged_dat,
…
Comment: gvcf joint calling
by
zihanss
•
0
Thank you for your help! I really appreciate it!
And you know, the merged WES gVCF files still have "NA" loci. For such cases, I am quite …
Comment: Correct way to compare multiple treaments between RNA-Seq samples using edgeR?
by
Guille
•
0
Thank you for your answer!
The MDS plot seems to group most treatment groups close-by, so I'm starting to suspect the effect of treatment …
Answer: Help understanding how KEGG Ortholog `K00004 ` has 3 ECs associated with it (EC:
by
Mensur Dlakic
★
27k
They say that we remember better things we figured out on our own, rather than things we were taught. You were literally two clicks away fr…
Comment: Heatmap and rna-seq
by
dsull
★
5.8k
https://maayanlab.cloud/clustergrammer/
It's a web-based tool (can also be a python jupyter widget) that I find useful for exploring a pat…
Answer: Limma Analysis Agilent Microarray Data (GPL1708)
by
Gordon Smyth
★
7.0k
There is an Agilent array case study in the limma User's Guide, which you might find helpful and somewhat simpler.
I don't recommend col…
Answer: Correct way to compare multiple treaments between RNA-Seq samples using edgeR?
by
Gordon Smyth
★
7.0k
The comparison you've done seems exactly right to test whether treatment 2 modifies or enhances the effect of treatment 1.
Have you exam…
Answer: How to use limma to find differentially expressed genes in response to a continu
by
Gordon Smyth
★
7.0k
There's no need for form a contrast because the score variable is already in the model. A continuous variable is its own contrast. Just run…
Comment: Create a new bed file with all pairwise combinations between two other bed files
by
J
▴
10
Oh wow, bedops closest-features, how did I overlook that!! I didn't know about it; thank you! Your awk script is beautiful. This worked per…
Comment: Create a new bed file with all pairwise combinations between two other bed files
by
J
▴
10
Thank you Pierre! I'll try this out with my full data set.
Answer: DNA methylation preprocessing
by
cao510927
▴
30
I happend to see one paper doing the similar thing, share it with you guys, "A systematic evaluation of normalization methods and probe rep…
Answer: Create a new bed file with all pairwise combinations between two other bed files
by
Alex Reynolds
35k
I think Pierre's answer might be correct for your test input, but it doesn't account for the start position of the reference element and so…
Comment: Highest variable features in single cell data
by
bk11
★
2.4k
You need to be clear about how `Seurat` defines highly variable genes here. Highly variable genes are the genes that have very high express…
Traffic: 1655 users visited in the last hour
Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by the
version 2.3.6
version 2.3.6