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20 results • Page
1 of 1
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Views
Votes
Replies
2
votes
4
replies
5.4k
views
Novaseq 6000 vs Novaseq X+
Illumina
NovaSeq6000
NovaSeqXPlus
sequencing
updated 16 hours ago by
GenoMax
150k • written 21 months ago by
KH
▴ 100
5
votes
11
replies
2.2k
views
6 follow
Trimming nanopore reads
trim
nanopore
updated 15 hours ago by
GenoMax
150k • written 14 months ago by
Nodilan
▴ 10
5
votes
6
replies
2.2k
views
benefit of DNA assembly
gene
genome
Assembly
sequence
updated 12 hours ago by
Ram
45k • written 10.9 years ago by
omid.omidi646
▴ 10
1
vote
4
replies
853
views
TaxID mapping file
annotation
blobtools
RNAseq
decontamination
transcriptomes
updated 15 hours ago by
WaspInSpace
• 0 • written 11 months ago by
Lada
▴ 40
1
vote
2
replies
615
views
QIAseq 16S/ITS Screening Panel Primers?
16s
primers
metagenomics
ITS
updated 10 hours ago by
GenoMax
150k • written 3.2 years ago by
Isaac
• 0
4
votes
3
replies
294
views
Why don't I see people calculating FPKM from normalized read counts?
normalization
RNA-seq
updated 6 hours ago by
kalavattam
▴ 330 • written 2 days ago by
shelkmike
★ 1.5k
1
vote
4
replies
288
views
Short reads to identify stuctural variants
SV
long-read
short-read
updated 15 hours ago by
GenoMax
150k • written 9 days ago by
priya.bmg
▴ 70
1
vote
3
replies
235
views
Trouble when uploading FASTQ files on NCBI
rnaseq
ncbi
19 hours ago by
Gerard
▴ 20
0
votes
0
replies
190
views
dittoHeapmap didn't work in my data set(error code :Error in .subscript.2ary(x, i, j, drop = TRUE) : subscript out of bounds)
dittoseq
scRNAseq
updated 10 hours ago by
ATpoint
87k • written 15 hours ago by
kayah
▴ 20
1
vote
3
replies
189
views
Different number of fragments after doublets removal in each run
ArchR
4 hours ago by
Bioinformatics_16
• 0
0
votes
5
replies
188
views
Picard : Bait_interval and Target_interval
Picard
Bed
read-depth
coverage
updated 8 hours ago by
Ram
45k • written 17 hours ago by
AIMAR
• 0
1
vote
3
replies
184
views
How to use LOEUF plugin in VEP?
LOEUF
VEP
updated 13 hours ago by
GenoMax
150k • written 1 day ago by
Sd
• 0
3
votes
2
replies
150
views
Does vg giraffe output secondary and supplimentary alignments?
vg
giraffe
23 hours ago by
lushjia
• 0
0
votes
1
reply
135
views
Analysing gene CNV from TCGA using TCGAbiolinks
TCGA
updated 11 hours ago by
Zhenyu Zhang
★ 1.2k • written 1 day ago by
Matt
• 0
0
votes
2
replies
119
views
Visualise duplications and inversions
inversions
duplications
updated 13 hours ago by
Wayne
★ 2.1k • written 15 hours ago by
priya.bmg
▴ 70
1
vote
1
reply
107
views
Exon-level counts via featureCounts
diffSpliceDGE
exon
RNASeq
featureCounts
edgeR
updated 13 hours ago by
GenoMax
150k • written 16 hours ago by
n_navy
• 0
0
votes
0
replies
99
views
Trouble with CIBERSORTx: All P-values > 0.05, Low Correlation, High RMSE – What Could Be Going Wrong?
R
CIBERSORTx
CIBERSORT
script
updated 13 hours ago by
GenoMax
150k • written 14 hours ago by
Pumla
• 0
0
votes
1
reply
90
views
How can I identify genes with the MSTRG notation?
R
stringtie
RNA-Seq
updated 14 hours ago by
GenoMax
150k • written 14 hours ago by
pinheirofabiano
▴ 110
0
votes
1
reply
78
views
UCSC's NCBI RefSeq Track tables: header differences
ucsc
refseq
browswer
genome
ncbi
updated 6 hours ago by
Pierre Lindenbaum
166k • written 7 hours ago by
Synanth
• 0
0
votes
0
replies
49
views
Issue with Agilent 2100 BioAnalyzer HS DNA Assay Trace
library-preparation
DNA
bioanalyzer
updated 8 hours ago by
Ram
45k • written 9 hours ago by
Beatrice
• 0
20 results • Page
1 of 1
Recent Votes
Answer: How to use LOEUF plugin in VEP?
A: How To Define Percent-Identity From A Cigar String/ A Bam/Sam File?
Comment: Different number of fragments after doublets removal in each run
Twice as many nFeature_RNA than nCount_RNA = more cell complexity? but how?
A: In Seurat, How Do nCount_RNA Differ from nFeature_RNA?
A: In Seurat, How Do nCount_RNA Differ from nFeature_RNA?
Answer: QIAseq 16S/ITS Screening Panel Primers?
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Comment: Different number of fragments after doublets removal in each run
by
Bioinformatics_16
• 0
Thank You for your response.
Comment: Different number of fragments after doublets removal in each run
by
Bioinformatics_16
• 0
###AddDoubleScores doubScores <- addDoubletScores( input = ArrowFiles, k = 10, #Refers to how many cells near a "pseudo…
Answer: UCSC's NCBI RefSeq Track tables: header differences
by
Pierre Lindenbaum
166k
My guess is that chromStarts plus blockSize is equivalent to exonStarts/exonEnds. If you need the bin column, you'll have to script things;…
Comment: Why don't I see people calculating FPKM from normalized read counts?
by
kalavattam
▴ 330
For anyone interested, this is the first paper to point out that RPKM (and, by extension, FPKM) normalization is inconsistent across sample…
Answer: QIAseq 16S/ITS Screening Panel Primers?
by
GenoMax
150k
From `Resources Tab` --> `Application Note` found at --> https://www.qiagen.com/us/products/next-generation-sequencing/metagenomics/targete…
Answer: Analysing gene CNV from TCGA using TCGAbiolinks
by
Zhenyu Zhang
★ 1.2k
I can not answer your TCGAbiolinks question, but I can answer your GDC CNV data question. There are two types of segment - float number, …
Comment: QIAseq 16S/ITS Screening Panel Primers?
by
microecologist93
▴ 20
Hello! Any luck figuring this out? I am now facing the same situation.
Comment: benefit of DNA assembly
by
Ram
45k
This post looks like it was generated using an LLM such as ChatGPT. If that is the case, please mention it in the comment. LLM generated co…
Comment: Picard : Bait_interval and Target_interval
by
AIMAR
• 0
Yes the genomic position like for the first line are not the same.
Answer: How to use LOEUF plugin in VEP?
by
GenoMax
150k
Looks like that file is available from Nature: https://static-content.springer.com/esm/art%3A10.1038%2Fs41586-020-2308-7/MediaObjects/41586…
Comment: Visualise duplications and inversions
by
Wayne
★ 2.1k
[SVbyEye](https://github.com/daewoooo/SVbyEye) says it handles duplications and inversions.
Comment: How to use LOEUF plugin in VEP?
by
Sd
• 0
Inside the loeuf.pm there is a link for the LOEUF scores that can be downloaded from https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7334197/b…
Comment: Visualise duplications and inversions
by
Medhat
9.8k
Maybe try samplot: https://github.com/ryanlayer/samplot
Comment: How can I identify genes with the MSTRG notation?
by
GenoMax
150k
More on MSTRG identifiers -- https://www.biostars.org/p/282817/#282832
Answer: Exon-level counts via featureCounts
by
GenoMax
150k
> I understand I need and exon-level count data Then you should use # Level of summarization -f Perform rea…
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