I'm relatively new to bioinformatics, and am trained primarily as a molecular biologist, so please bear with me if my questions seem quite rudimentary.
I've received a dataset of RNA-sequence data that has already been converted from its native form to an Excel spreadsheet. It contains data from 2 conditions, one experimental and one control. Each is done in replicate, and both the raw values and the log transformed values are present. I'm trying to utilize this data to compile a list of differentially expressed genes, so my questions are as follows:
1) Is there a way in Excel where I can isolate the differentially expressed genes from the large dataset that I have now? Is there a way on Excel to statistically analyze the difference in expression of each gene, and only isolate the ones which have a difference in expression of p<0.05?
2) From this dataset, is there a way on Excel to individually isolate the genes which have been up-regulated and down-regulated separately?
Again, I apologize if these questions are basic, and would greatly appreciate any assistance from the community.