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25

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11

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3.7k

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6 follow

meta biostars Guidelines

updated 2 days ago by Ram 43k • written 14.6 years ago by Istvan Albert 100k

3

votes

3

replies

214

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PC

updated 2 days ago by GenoMax 141k • written 2 days ago by Estevão • 0

0

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0

replies

158

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annotation scRNA llm

2 days ago by yxwucq • 0

3 results • Page 1 of 1

Recent Votes

Answer: Seurat merge and batch correction

Comment: How to convert plink files to Hapmap Format

Answer: Missing protein (VEGF-A) in String db

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Recent Replies

Comment: Missing protein (VEGF-A) in String db by shalespringer • 0

Thank you for replying here; this helped me figure out why MAPK10 was missing from my results. It was also marked as a pseudogene in the En…

Comment: How to convert plink files to Hapmap Format by Sofia • 0

These are the first lines of the output: (Please is it normal to have NA in the P value column ?) CHR …

Comment: How do I use the STARSolo aligner with MGI DNBelab C series HT scRNAseq librarie by atowns21 • 0

So I used the barcodes that I created (combos of positions 1-10 and 11-20) and I obtained similar alignment stats as the paper I pulled the…

Comment: How to convert plink files to Hapmap Format by Sofia • 0

Thank you so much, it actually worked!

Answer: Rare Disease Variant Pathway Analysis by LauferVA 4.2k

Hi @efc1e545 , First a caveat. the information we most need in order to help guide you to a successful conclusion is not provided in thi…

Answer: Sequence read length shorter than flow cell specification by swbarnes2 14k

The company probably had you share your run with someone who needed the extra bases. So you get the extra bases free. Just use them unle…

Comment: how to combine multiple RNAseq count files into a single dataframe in R and unix by Ram 43k

Thank you, the `csvtk spread` is super useful. I usually import into R using `lapply` then `Reduce` using `merge` but this might be easier.

Comment: Annovar using R package by DKA ▴ 40

Thank you for your guidance. The thing is that I am unfamiliar with using such environments, unfortunately.

Comment: Treatment VS Control in Single Cell RNAseq analysis by ATpoint 82k

Open a new question, with details.

Comment: Normalize scRNAseq data to housekeeping genes to compare several datasets by ATpoint 82k

I recommend https://bioconductor.org/books/3.18/OSCA.basic/normalization.html as well as the "advanced" section in this book.

Comment: Why gatk VariantAnnotator required bam and coverage files by QX • 0

thank you!

Comment: Treatment VS Control in Single Cell RNAseq analysis by kilcdincer ▴ 10

Hello, I have more or less same experimental setting and was wondering how you proceeded with your analysis? Can I reach its GitHub reposit…

Comment: BLAST using both nucleotides and taxonomic local databases by GenoMax 141k

> I ask this as some BLAST command fields as scinames or sblastnames do not give any output with a classic nt BLAST If that information is…

Answer: BLAST using both nucleotides and taxonomic local databases by 5heikki 11k

This has been discussed [many times][1] [1]: https://www.biostars.org/p/76551/

Answer: why renaming Idents in Seurat object doesn't work? by Bioinfotec ▴ 10

I think when you give indent to seuObj : ```r Idents(seuObj) <- 'RNA_snn_res.0.1' ``` You may wrongly assign other value to it such as `…

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