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Thank you for your explanation. I am working on the cell lines and I am investigating the effect of the treatment on the expression level, …
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Not sure I follow. Can you first of all describe which comparisons you want to make, pretending there was no batch effect at all. Write dow…
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Thank you for the advice, i'll keep that in mind.
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Most likely you didn't do anything wrong. Going by your previous post that you have only 8 GB of RAM, the most likely reason is the lack of…
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Hey! Can you explain the question further? What have you tried?
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I tried the 2nd option only.
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Isn't the download link available above the plot: http://bis.zju.edu.cn/HCL/data/DGE/Adult-Transverse-Colon1_dge.txt.gz
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You need to change this configuration file to point it to the location where the aligner executable can be found.
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The expression levels do not change but the way that you have parametrized the expression levels does change.
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